NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312). Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.
A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
2D gel stained with the SYPRO Ruby protein gel stain and the Pro-Q Emerald 300 reagent. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›
Processing time and protein recovery using 20-100 mL protein concentrators Go ›
Processing time and protein recovery using 5-20 mL concentrators Go ›
Performance of the Qubit® dsDNA HS Assay Go ›
Low protein-to-protein variation in the Qubit® protein assay Go ›