Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator.
Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator (M36008). Live 3T3 cells were treated with FeTCPP, a superoxide scavenger, (bottom) or left untreated (top). Cells were then labeled with MitoSOX™ Red reagent, which fluoresces when oxidized by superoxide, and nuclei were stained with blue-fluorescent Hoechst 33342. The mitochondria of untreated cells exhibited red fluorescence, indicating the presence of superoxide, whereas the mitochondria of treated cells showed minimal fluorescence.
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›
Oxidative stress analysis in BPAE and Raw cells using CellROX® Deep Red Reagent (Cat. No. C10422) Go ›
BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) Go ›
Agarose gel containing camptothecin-treated HL-60 cells. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›