Sample Preparation Assays for Flow Cytometry

Careful preparation makes the difference

Quality data requires quality starting material. Achieve the best possible results with highly referenced sample preparation reagents for flow cytometry including intracellular and transcription factor staining buffer sets, cell lysis and preservation assays, blocking reagents, and magnetic cell isolation beads.

Flow cytometry buffer selection

  Cal-Lyse Solutions High-Yield Lysis Solutions
Use
  • Premixed lysis solution formulated to lyse erythrocytes following monoclonal antibody staining of whole blood or bone marrow


Learn more about cell preservation and lysis buffers
  • Premixed, fixative-free erythrocyte-lysis solution that can be used to help eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations
Learn more about cell preservation and lysis buffers
Contents
  • Whole blood lysis buffer
  • Formaldehyde fixative
  • Fixative-free whole blood lysis buffer
Size 25 mL 100 mL 500 mL
Cat. No. GAS010 GAS010S100 HYL250
Regulatory status CL1 – Class I Device CL1 – Class I Device
When to use

Intended Use Statement:
For In Vitro Diagnostic Use.

Intended to be used as an aid in the enumeration of leukocytes from various human biological sources including blood and bone marrow that have been stained with monoclonal antibodies for analysis using flow cytometric methods.

Intended Use Statement:
For In Vitro Diagnostics Use.

Intended to be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations.
  eBioscience Flow Cytometry Staining Buffer
Use
  • For antibody and cell dilution steps, as well as wash steps required for surface staining and flow cytrometric analysis
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the Flow Cytometry Staining Buffer
Contents Buffered saline solution:
  • Fetal bovine serum
  • Sodium azide (0.09%) as a preservative
Size 200 mL
Cat. No. 00-4222-57
  eBioscience Intracellular Fixation & Permeabilization Buffer Set FIX & PERM Cell Fixation & Cell Permeabilization Kit
Use
  • Stain both cytoplasmic proteins as well as proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates


Learn more about the IC Fix & Perm buffer set
  • Use when staining intracellular markers, such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, and immunoglobulins
  • Mild fixation and permeabilization of cells that leaves their morphological scatter characteristics intact
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the FIX & PERM Kit
Contents
  • IC Fixation Buffer
  • Permeabilization Buffer (10X)
  • Fixation Medium (Medium A)
  • Permeabilization Medium (Medium B)
Size 1 kit 50 tests
Cat. No. 88-8824-00 GAS003
Regulatory status RUO – For Research Use Only. Not for Use in Diagnostic Procedures. GPR – General Purpose Reagent.
When to use For use in research only (i.e. research on any sample type). For use in general laboratory application, that is used to collect, prepare, and examine specimens from the human body for diagnostic purposes.
  eBioscience Foxp3 / Transcription Factor Staining Buffer Set
Use
  • Formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67
  • Also applicable to simultaneous nuclear and cytoplasmic (cytokines and transcription factors) staining
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the Foxp3 / Transcription Factor Staining Buffer Set
Contents
  • Fixation/Permeabilization Concentrate
  • Fixation/Permeabilization Diluent
  • Permeabilization Buffer (10X)
Size 1 kit
Cat. No. 00-5523-00
  eBioscience Super Bright Staining Buffer
Use
  • Use when more than one Super Bright dye–conjugated antibody is present in an experiment. This buffer helps prevent non-specific polymer interactions, which can result in data appearing under-compensated
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn about Super Bright Antibody Conjugates
Contents
  • Super Bright Staining Buffer
Size 100 tests
Cat. No. SB-4400-42
  Cal-Lyse Solutions High-Yield Lysis Solutions
Use
  • Premixed lysis solution formulated to lyse erythrocytes following monoclonal antibody staining of whole blood or bone marrow


Learn more about cell preservation and lysis buffers
  • Premixed, fixative-free erythrocyte-lysis solution that can be used to help eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations
Learn more about cell preservation and lysis buffers
Contents
  • Whole blood lysis buffer
  • Formaldehyde fixative
  • Fixative-free whole blood lysis buffer
Size 25 mL 100 mL 500 mL
Cat. No. GAS010 GAS010S100 HYL250
Regulatory status CL1 – Class I Device CL1 – Class I Device
When to use

Intended Use Statement:
For In Vitro Diagnostic Use.

Intended to be used as an aid in the enumeration of leukocytes from various human biological sources including blood and bone marrow that have been stained with monoclonal antibodies for analysis using flow cytometric methods.

Intended Use Statement:
For In Vitro Diagnostics Use.

Intended to be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations.
  eBioscience Flow Cytometry Staining Buffer
Use
  • For antibody and cell dilution steps, as well as wash steps required for surface staining and flow cytrometric analysis
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the Flow Cytometry Staining Buffer
Contents Buffered saline solution:
  • Fetal bovine serum
  • Sodium azide (0.09%) as a preservative
Size 200 mL
Cat. No. 00-4222-57
  eBioscience Intracellular Fixation & Permeabilization Buffer Set FIX & PERM Cell Fixation & Cell Permeabilization Kit
Use
  • Stain both cytoplasmic proteins as well as proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates


Learn more about the IC Fix & Perm buffer set
  • Use when staining intracellular markers, such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, and immunoglobulins
  • Mild fixation and permeabilization of cells that leaves their morphological scatter characteristics intact
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the FIX & PERM Kit
Contents
  • IC Fixation Buffer
  • Permeabilization Buffer (10X)
  • Fixation Medium (Medium A)
  • Permeabilization Medium (Medium B)
Size 1 kit 50 tests
Cat. No. 88-8824-00 GAS003
Regulatory status RUO – For Research Use Only. Not for Use in Diagnostic Procedures. GPR – General Purpose Reagent.
When to use For use in research only (i.e. research on any sample type). For use in general laboratory application, that is used to collect, prepare, and examine specimens from the human body for diagnostic purposes.
  eBioscience Foxp3 / Transcription Factor Staining Buffer Set
Use
  • Formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67
  • Also applicable to simultaneous nuclear and cytoplasmic (cytokines and transcription factors) staining
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn more about the Foxp3 / Transcription Factor Staining Buffer Set
Contents
  • Fixation/Permeabilization Concentrate
  • Fixation/Permeabilization Diluent
  • Permeabilization Buffer (10X)
Size 1 kit
Cat. No. 00-5523-00
  eBioscience Super Bright Staining Buffer
Use
  • Use when more than one Super Bright dye–conjugated antibody is present in an experiment. This buffer helps prevent non-specific polymer interactions, which can result in data appearing under-compensated
  • Use with any cell type (human, mouse, etc.)
  • Volume can be scaled for use in both test tubes and plates
Learn about Super Bright Antibody Conjugates
Contents
  • Super Bright Staining Buffer
Size 100 tests
Cat. No. SB-4400-42

Cell isolation and expansion reagents

  Dynabeads for cell isolation MagniSort Cell Separation Technology
Use
  • Cell isolation
  • Cell activation and expansion
  • Clinical and non-clinical research
  • Cell isolation, including cell enrichment and depletion
Technology Dynabeads activation and expansion technology mimics in vivo T cell activation.

Available for human and mouse basic research as well as clinical research settings.

Learn more
Starting with T cell–specific cocktails, use the MagniSort platform to obtain enriched T cells simpler, faster and with significant cost-savings compared to traditional column-based separation methods.

Learn more

Resources

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builderbeta—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Protocols

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