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Invitrogen
Rat anti-Mouse IgG1 Secondary Antibody, FITC
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FIGURE: 1 / 1
Mouse IgG1 Secondary Antibody (RMG101) in ICC/IF
Immunofluorescence analysis of Rat anti-Mouse IgG1 Secondary Antibody, FITC was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 h... View More
Product Details
RMG101
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
0.2 µg/mL
Product Specifications
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
LO-MG1
Immunogen
IgG gamma 1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.4 mg/mL
Amount
200 µg
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Target
IgG1
Antibody Form
Whole Antibody
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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