Efficient. Flexible. Transformative.

The Attune NxT system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; field upgradeable
  • Transformative—run difficult samples with a system that is less sensitive to clogging
     

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快速且不堵的流式细胞仪

  • 堵塞问题迎刃而解 - 专业抗堵塞设计,轻松检测其它流式细胞仪不能检测的大体积、粘性样本
  • 节省10倍时间 - 上样速度提高10倍,但丝毫不损失数据精确度
  • 简化样品制备 - 提供全血免洗免裂解方案
  • 自动化无人值守 -简单、快速的微孔板/试管转换
  • 随时根据您的需求对系统进行升级 - 模块化设计,根据您的经费灵活升级

非一般的流式体验

样本检测速度更快、精度更高 - 无需担心因堵塞而损失样本

Invitrogen Attune NxT流式细胞仪是一台精确性和性能完美结合的纯正台式流式细胞仪,最多可配置4色激光和16个检测通道。该细胞仪以消除流式细胞仪痼疾,推动科学新发现为使命。例如,其可支持对更多类型的样本进行更强大的分析,包括癌症细胞样品。结合Invitrogen Attune自动上样仪使用,有助于大幅提高高通量多参数筛选速度。

堵塞问题终于迎刃而解

Attune NxT流式细胞仪为肿瘤细胞、心肌细胞、贴壁细胞等类型细胞应用引入了抗堵塞设计。

  • 避免仪器堵塞引起的困扰、沮丧和样本损失
  • 可分析难检测样本和体积大、粘性的细胞,包括以前不能用流式检测的肿瘤细胞、心肌细胞和脂肪细胞。
流式细胞仪过去并不能很好的检测体积大的粘性样本,往往会因为系统堵塞导致样本损失、项目延期,并且需要严密监控设备运行。Attune NxT流式细胞仪可以轻松检测其它流式细胞仪不能检测的大体积、粘性样本,包括肿瘤样本、心肌细胞、脂肪细胞以及以前不能检测的多种类型细胞,从而可对多种细胞类型进行高通量统计学分析。

节省时间 – 速度提高10倍,但丝毫不损失数据精确度

过去需要花一整天完成的实验,现在仅需几小时。Attune NxT流式细胞仪可助力您的科学研究提高采样速率,不但能帮您加速完成实验,更可让真正的科学发现触手可及。不要再让样本待在在冰箱里蹉跎,立刻开始分析吧。

  • 真正的35000细胞/秒的读取速度和1 mL/min的流速,不会丢失任何数据,采集传输速度相当于同时播放10部高清电影
  • 声波聚焦技术、完美的液流设计、优化的光学配置,以及高速的数据传输,让Attune NxT流式细胞仪的速度比传统流式细胞仪快十倍
  • 样本检测时间只有市面上大部分仪器的几分之一
  • 可对难检测样本进行稀释,从而获得更多研究所需的数据
  • 轻松“大海捞针”,找到新的细胞类群(点击查看类似研究
  • 可分析您以前不能分析的细胞类型

Attune NxT实现了超高的样本通量

Attune NxT声波聚焦流式细胞仪采用声波辅助流体聚焦技术,可实现12.5 µL/min–1 mL/min或12.5–1,000 µL/min的样本运行速率 – 速度高达传统流体聚焦系统的10倍,同时数据采集速率达到35,000事件/s。

相对于其他仪器,Attune NxT声波聚焦流式细胞仪仅需后者几分之一的时间即可采集1,000,000个事件。图中的数据表示在各个仪器的最大采样速率(Attune NxT 声波聚焦流式细胞仪为1,000 µL/min)下的实验方案耗时。

查看更多数据

采用Attune®NxT流式细胞仪,可在高进样速率下最大程度地减小数据差异。 对Jurkat细胞进行固定,RNA酶处理,并用碘化丙啶(PI)染色,制成浓度为1 x 106个细胞/mL的溶液,以不同的进样速率在Attune® NxT声波聚焦流式细胞仪上进行分析。不论进样速率如何,Attune® NxT流式细胞仪上G0/G1和G2/M峰的宽度以及CV%都很稳定,即便是在1,000 μL/分钟的最高进样速率下也是如此。

根据您的需求对系统进行升级

模块化设计,可配置4种激光和14色荧光

Attune NxT Acoustic Focusing Cytometry --“Meet the Inventor Series “Greg Kaduchak Interview

Invitrogen™ Attune™ NxT声波聚焦流式细胞仪能够兼容流式细胞仪中最常用的荧光基团和荧光蛋白,可以与您目前运行的检测试剂盒匹配。采用可选的561 nm激光还可检测多种荧光蛋白 (图2)。无论您要立即配置自己的系统还是要等待一段时间再升级,Attune NxT声波聚焦流式细胞仪均可与您一道成长,满足您的研究需求。

Attune NxT流式细胞仪配置选项

激光 激光配置(货号) 所含的激光,检测通道数 全部检测通道*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
3 Blue/green/red
(A28999)
Available as upgrade†
3
 
4
3
12
Blue/green/violet
(A28997)
4
3
 
4
Available as upgrade† 13
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
*检测通道数包括所有荧光通道和前向角以及侧向角通道。
† Attune NxT流式细胞仪用户如需望购买激光升级服务,请点及此处询价:黄色激光升级紫色激光升级红色激光升级绿色激光升级。在收到您的询价请求后,我们的技术销售专员会和您联系商定升级选项事宜。

简化了样品制备 – 无需洗涤、无需裂解选项。

声波聚焦技术可以大幅提升采样速率,简化了样品制备过程、缩短了实验方案耗时,最大程度地避免了细胞损失。该特性尤其适合于采用全血进行的无需洗脱/裂解的免疫表型分型实验方案,以及浓度一贯较低的样本,包括脑脊液和干细胞制备物。

Acoustic focusing allows significantly higher sample rates

Attune NxT Flow Cytometer is able to perform immunophenotyping analysis on minimally processed samples, reducing your 10-step sample prep protocol to 3 easy steps.

查看更多数据

无人值守自动运行,简单、快速的平板/试管转换

配有自动上样器的Attune NxT流式细胞仪,为流式细胞分析引入多参数筛选能力,可对更多类型的细胞和细胞系进行高通量分析。

  • 灵活 - 可读取96孔板和384孔板,包括容积达2 mL的深孔板
  • 简单 - 仅需点击鼠标,即可在几秒内轻松实现细胞反应板和试管之间的转换,无需硬件替换或关机重启 -其它系统需要30-45分钟的停机时间。
  • 解放实验室人力资源 - 设计实现了无人值守性能,装备了抗堵塞鞘液和软件

可实现独特的应用

您的下一步结果依靠的是您的试验设计及仪器提供更多信息的能力 – 提供更多的数据、更多的详情、更大的通量。Attune NxT声波聚焦流式细胞仪可满足更为复杂的试验要求,能够日复一日地提供可靠的数据,与您的系统一道成长。

See the data

  • Software for the Attune NxT Flow Cytometer ›
  • Optics
    • Laser power (as shown in table below)
      Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
      Violet 405 50 100
      Blue  488 50 100
      Green  532 100 140
      Yellow 561 50 100
      Red 637 100 140
      * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
      ** Vendor-specified theoretical maximum.
    • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection 
    • Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment 
    • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 
    • Laser separation: 150 μm 
    • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
    • Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off 
    • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage 
             ––Flat top specified at the flow cell; CV <3% over width of flat top
             ––Field upgradable
    Fluidics
    • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
    • Sample analysis volume: 20 μL to 4 mL
    • Custom sample flow rates: 12.5–1,000 μL/min
    • Sample delivery: Positive-displacement syringe pump for volumetric analysis
    • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
    • Fluid-level sensing: Active
    • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
    • Fluid storage: All fluids stored within instrument
    • Extended fluidics option: Configuration for 10 L fluid
    • Nominal fluid consumption: 1.8 L/day
    • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
    Performance
    • Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
    • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
    • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
    • Maximum electronic speed: 65,000 events/sec with all parameters
    • Carryover: Single-tube format: <1%
    • Forward and side scatter sensitivity: Able to discriminate platelets from noise
    • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
    • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
    • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
    • Fluorescent detectors: 14 individual detectors
    • Electronic pulse: Measured area, height and width pulse for all detectors
    • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
    • Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories
    Software
    • Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
    • Flow rate: Precise flow rate control via software; no hardware adjustments
    • Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
    • Overlays: Comparative analysis between samples; 3D view
    • Sample recovery: System able to return unused samples
    • Concentration: Direct concentration measurement without use of counting beads
    • Software layout: Fully customizable for each user account
    • Bubble detection technology: Stops automated run to preserve sample integrity
    • Maximum single-event file: 20 million with option to append
    • Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
    • Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
    • Gating: Hierarchal gating with the ability to derive gates
    • Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
    • Voltage: User adjustable
    • Window extensions: User adjustable
    • Area scaling factor (ASF): User adjustable
    • Acquisition settings: Documented in FCS files and maintained upon import
    • Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
    • Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
    • Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
    • User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
    Quality and regulatory
    • Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
    • Warranty: 1 year
    • Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
    • Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
    • Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
    • For Research Use Only
    Data management
    • Software requirements: Invitrogen Attune NxT Software
    • Monitor: 23-inch flat panel (1,920 x 1,200 resolution); dual-monitor capability
    • Computer: Minitower desktop
    • Operating system: Windows™ 7 64-bit
    • FCS format: FCS 3.1, 3.0
    • Processor: Intel Core™ i7 processor
    • RAM: 16 GB
    • Hard drives: 80 GB or larger and 250 GB RAID-compatible hard drives
    Installation requirements
    • Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
    • Heat dissipation: <150 W
    • Temperature operating ranges: 15–30°C (59–86°F)
    • Operating humidity: 10–90%, noncondensing
    • Audible noise: <65 dBA at 1.0 m
    • Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
    • Weight: ~29 kg (64 lb)
    • Available configurations (see Attune NxT Flow Cytometer configuration options table)

    Simple yet powerful software, easy auto compensation

    Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

    • Compensation—fully automated and manual compensation
    • Instrument tracking—automated baseline and performance test for all channels and linearity
    • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
      monthly decontamination
    • User account maintenance—administrative and individual accounts with user log
    • Gates—standard and customizable gates
    • Plot previews—easy workspace setup
    • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
    • File formats—FCS 3.0 and FCS 3.1
    • Graphics resolution—publication-quality images (customizable)

    Learn more about software for the Attune NxT Flow Cytometer

    Scientific posters

    High Sensitivity Applications for the Attune Acoustic Focusing Cytometer
    Presented by Bill Telford, head of the Experimental Transplantation and Immunology Branch's FACS Core Facility at CYTO 2012.
    Studying Malaria using the Attune Acoustic Focusing Cytometer
    Presented by Grace Chojnowski, Flow Cytometry and Imaging Facility manager at Queensland Institute of Medical Research at CYTO 2012.
    A Novel No-Wash, No-Lyse Method For Residual Leukocyte Enumeration Using Acoustic Focusing Flow Cytometry and a Cell-Membrane Permeant DNA Dye
    Presented by José-Enrique O'Connor, head of the Laboratory of Cytomics at Centro de Investigación Príncipe Felipe at CYTO 2012.
    Ultrasmall And Beyond: Nuclear Holopoloid Genome Sizing of Carnivorous Plant Species by Acoustic Focusing Cytometry
    Flow cytometry has been widely utilized as an expeditious and accurate method for the estimation of plant holoploid nuclear genome size (C‐value).
    Phospho‐Protein Detection Using Acoustic Cytometry
    Multiparameter flow cytometry is an important tool for examining cell signaling pathways. Using this acoustic focusing technology the sample stream can be slowed down...
    Acoustic Focusing Cytometry: Sensitivity and Throughput
    We examined changes in sensitivity with sample flow rate using acoustic focusing cytometry. We performed typical assays using the Applied Biosystems Attune Acoustic Focusing Cytometer...
    Acoustic Flow Cytometry for Multicolor Immunophenotyping Analysis
    Acoustic focusing enables both longer cell interrogation times and higher throughput, which provide higher-precision analysis of dilute samples without compromising the speed of collection...
    Identification and Initial Characterization of Limbal Stem Cells Isolated From Human Cornea
    Limbal stem cells are known to be precursor cells that repopulate the cornea during wound healing and corneal repair. Limbal stem cells in the basal region give rise to transit amplifying (TA) cells...
    Rare Event Detection using Acoustic Cytometry
    Detection of rare events (defined as populations of cells that make up less than 5% of the total cells), includes the detection of stem cells, minimal residual disease, tetramers, NKT cells, and fetomaternal...
    Acoustic Cytometry for Rare Event Detection of PNH Cells
    Flow cytometry is the method of choice for detection of paroxysmal nocturnal haemoglobinuria (PNH), and is made possible with technological improvements in instrumentation combined with...

    服务计划

    您可以优化自己的仪器性能,最大限度发挥关键系统的利用效率,简化您的实验室工作流程。只需为您的Attune NxT流式细胞仪选择最适合您的实验室需求和预算的支持水平。

    • AB Complete Plan
    • AB Assurance Plan
    • AB Maintenance PLUS Plan

    耗材和附件

    除了样品加样其等附件外,还可找到操作您的Attune NxT流式细胞仪所需的各种溶液,包括

    • Attune聚焦流体
    • Attune洗涤溶液
    • Attune置换溶液
    • Attune性能示踪微珠

    Flow Panel Design Tool
     

    Choose antibody conjugates for your panel in 3 steps: 1) pick the antibody species reactivity, 2) select up to 14 targets, and 3) choose the lasers or fluorophores you want to view.

    Fluorescence SpectraViewer

    This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.

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