Xcalibur Data Acquisition and Interpretation Software

Acquire high-quality data, regardless of your mass spectrometry platform

Confidently control, acquire, and interrogate data from a range of Thermo Scientific LC-MS systems and related instruments using Thermo Scientific Xcalibur software.

With simple method set-up, data acquisition, in-depth data interrogation tools as well as seamless integration to a range of additional Thermo Scientific application-specific software and cloud-based tools, Xcalibur software serves as the powerful, yet easy-to-use platform for your LC-MS analyses.

Key benefits of Xcalibur data acquisition software

Comprehensive toolset and landing page for all your installed applications

Ad-hoc data reviewing and quantitative capabilities

Integration with cloud-based tools for productivity

With a homepage that conveniently hosts all your installed applications and provides easy access to instrument control, method development, data acquisition, and reviewing. Xcalibur software makes it quick and easy to navigate through your workflows.

When you need to interrogate your data—from elemental compositions, plotting chromatograms, spectral views, performing library searches and much more—or perform basic quantitation, the Thermo Scientific FreeStyle application included with the Xcalibur software allow you to gain insights into your data.

The landing page for the Xcalibur software not only serves as the configurable portal to your applications, you can also receive application update information, to help ensure that you have the latest version of software.

Learn more about Xcalibur software and its data interrogation tools

The customizable landing page (or Roadmap View) enables easy access to all your installed applications, from those included with Xcalibur software to your application-specific software packages.

Not only can you receive notification when there is a new version of any installed application, you can also access the XApp Store for more information on the latest applications and try many of them for free for 60 days.

For Thermo Scientific Ardia Platform users, Xcalibur software provides Ardia Empower Workflows so that users can directly save acquired data in the Ardia Repository. In addition, Xcalibur software could automatically trigger Thermo Scientific Proteome Discoverer data processing for these type of submissions. The Ardia Empower Workflows allows open collaboration, and efficient easy data sharing among the Ardia Platform users via the Ardia data repository.

Regardless of the experimental design, it should be easy to set up your mass spectrometer to acquire high-quality data. The Method Editor allows you to quickly and simply create acquisition methods that control the necessary parameters and drive your acquisition requirements.

For the latest generation of mass spectrometers, there are numerous pre-configured method templates arranged by application area. These methods allow users of any experience level to acquire data relevant to their questions. Alternatively, the templates can be used as a starting point to facilitate rapid method development. Each method covers all instrument parameters as well as those for data acquisition, reducing complexity of set up.

Modern visualization tools allow you to rapidly answer questions as you qualitatively interrogate your data.

The FreeStyle application provided with the Xcalibur software, allows you to interact with your data from simple chromatographic detection and integration to spectral library searching, spectral deconvolution or diagnostic plots.

For the qualitative analysis of any mass spectral data (.raw or .sld) in a highly visual environment, FreeStyle allows you to display chromatograms and spectra, detect and integrate chromatographic peaks, search mass spectral libraries, simulate mass spectra, subtract background spectra, apply scan filters, annotate plots with text and graphics, create and save layouts, and view the status of various instrument parameters during data acquisition.

Download FreeStyle

With a familiar interface, the tools needed to access the information stored in your data are readily accessible. HRAM data intelligence means that data is displayed according to the mass accuracy of the MS system used to acquire it.

When it comes to visualizing your data, make use of unlimited plots, quick creation of plots from spectra, spectral annotation with structures, text and graphics, trending/diagnostic plots for status parameters, and scan header entries; float/dock/hide multiple windows and split across monitors to really get the most out of your data.

Create and customize highly visual map views of complex datasets, with simple modification of any plots and data views. Then use those views to generate your own visual reports, enhancing your insights.

High-resolution protein mass spectral data can be deconvoluted using the Xtract deconvolution algorithm to obtain the intact mass and a single, deconvoluted mass spectrum for the protein components within the charge state envelope.

Discovering what is in your data doesn’t need to be challenging. The ability to search the Thermo Scientific mzCloud mass spectral library, Thermo Scientific mzVault or NIST with a single-click means that compound identification from MS and MS2 data is quick and easy. You can also export spectral data to the NIST application.

Elemental compositions can be calculated using MS and MS² data, with isotopic simulations for added confidence.