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Harness the power of dPCR for precise and sensitive absolute quantification
Digital PCR (dPCR) is a leading technology for detection and quantification of rare mutations and target sequences. Overcome challenges with other ddPCR and dPCR platforms and leverage the full power of dPCR with the Applied Biosystems QuantStudio Absolute Q Digital PCR System, featuring:
- Broad application support backed by our TaqMan Assay portfolio
- Proprietary technology with industry-leading accuracy and consistency
- Super-simple workflow with fast time to answer
- Modular automation setup for flexible high-thoughput workflows
dPCR vs qPCR—what is the difference?
Both digital PCR (dPCR) and real-time PCR (qPCR) can be used to detect and quantify nucleic acids. While these technologies share similarities, key differences in quantification methods confer each tool with application-dependent benefits or limitations.
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Applications for dPCR
Explore an array of dPCR applications to suit your research needs.
Additional applications
NGS library quantification and verification
dPCR provides precise quantification of next-generation sequencing libraries to help maximize data quality and output. Because no other samples or standard curves need to be run, dPCR removes a source of variability to help ensure accurate results.
Gene editing
Detect genome editing events in a fast and reliable way, especially those created using nucleases that cause double-stranded breaks, such as CRISPR-Cas9. Precise quantification of these nucleic acid editing events is crucial to determine the activity and efficiency of these enzymes in cells.
Webinar: A digital PCR-based protocol to detect and quantify RNA editing events at hotpots
Rare mutation detection
Detection of rare mutations can be particularly challenging because probes for mutants usually only differ from wild type by a single base. dPCR overcomes the challenge of probe-sequence similarities by digitizing into micro-reactions, thereby enriching for the targets. It is 100-times more sensitive than conventional methods for this type of analysis, with the ability to pool samples for even higher sensitivity.
Technical Note: Rare target quantification on the QuantStudio Absolute Q dPCR
Absolute Q Digital PCR assays and reagents
Microfluidic array plate technology
Not all dPCR is created equal. Our microfluidic array plate (MAP) technology offers significant benefits over droplet digital PCR (ddPCR) technologies, including:
- Superior consistency and accuracy
- Reduced dead volume (<5%)
- Simpler workflow
- Faster time to answer
Resources
Taq Academy
Expand your digital PCR expertise with Taq Academy's webinars, technical literature, and virtual and in-person events.
Absolute Gene-ius podcast
Check out our podcast, where we interview trailblazing scientists about their career journeys, lab stories and groundbreaking digital PCR research.
Talk to a specialist
Have questions about implementing dPCR in your lab? Let us help you get started!
For Research Use Only. Not for use in diagnostic procedures.