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CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE, eBioscience™
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FIGURE: 1 / 5
CD154 (CD40 Ligand) Antibody (12-1548-42) in Flow
Human peripheral blood cells unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) were stained with Anti-Human CD8a FITC (Product # 11-0088-42) and Anti-Human CD154 (CD40 Ligand) PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-1548-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
24-31
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.
Applications Reported: 24-31 has been reported for use in flow cytometric analysis.
Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of activated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5; unnamed protein product
Gene Aliases: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
UniProt ID: (Human) P29965
Entrez Gene ID: (Human) 959, (Cynomolgus monkey) 102138630
regulation of immunoglobulin production
regulation of immune system process
inflammatory response
immune response
cell communication
leukocyte cell-cell adhesion
cell surface receptor signaling pathway
integrin-mediated signaling pathway
regulation of gene expression
CD40 signaling pathway
signaling
platelet activation
B cell differentiation
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-4 production
B cell proliferation
positive regulation of T cell proliferation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
isotype switching
positive regulation of NF-kappaB transcription factor activity
positive regulation of endothelial cell apoptotic process
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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