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CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eBioscience™
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FIGURE: 1 / 4
CD154 (CD40 Ligand) Antibody (14-1548-82) in Flow
Cells 2023 -
Production of CD40L KO and GM-CSF KO CAR T cells using CRISPR-Cas9 technology. ( A , B ) Target sites. Schematically shown are the CD40L and CSF2 loci with the respective numbers of exons and the corresponding CRISPR-Cas9 target sites (protospacer in purple, PAM in green). ( C , D ) Phenotype. On day 8 after electroporation expression levels of CD40L ( C ) or GM-CSF ( D ) were determined using flow cytometr... View More
Product Details
14-1548-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Published species
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
24-31
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.
Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro. (Please use Functional Grade Purified 24-31, Product # 16-1548, in functional assays).
Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5; unnamed protein product
Gene Aliases: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
UniProt ID: (Human) P29965
Entrez Gene ID: (Human) 959, (Cynomolgus monkey) 102138630
regulation of immunoglobulin production
regulation of immune system process
inflammatory response
immune response
cell communication
leukocyte cell-cell adhesion
cell surface receptor signaling pathway
integrin-mediated signaling pathway
regulation of gene expression
CD40 signaling pathway
signaling
platelet activation
B cell differentiation
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-4 production
B cell proliferation
positive regulation of T cell proliferation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
isotype switching
positive regulation of NF-kappaB transcription factor activity
positive regulation of endothelial cell apoptotic process
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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