Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), PE, eBioscience™

11 Published Figures
21 References
View all (59) CCR7 antibodies

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Product Details

12-1979-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 21 publications 21 publications
Product Specifications

Species Reactivity

Human

Published species

C. elegans, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670625

Product Specific Information

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

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UniProt ID: (Human) P32248

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Entrez Gene ID: (Human) 1236

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Function(s)
G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity
Process(es)
establishment of T cell polarity positive regulation of cell-matrix adhesion dendritic cell chemotaxis myeloid dendritic cell chemotaxis positive regulation of dendritic cell antigen processing and presentation positive regulation of hypersensitivity positive regulation of humoral immune response inflammatory response immune response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of actin filament polymerization positive regulation of pseudopodium assembly ruffle organization response to lipopolysaccharide regulation of interferon-gamma production positive regulation of interleukin-12 production response to prostaglandin E chemokine (C-C motif) ligand 19 signaling pathway chemokine (C-C motif) ligand 21 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of phosphatidylinositol 3-kinase activity negative thymic T cell selection positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of JNK cascade homeostasis of number of cells regulation of interleukin-1 beta secretion positive regulation of T cell receptor signaling pathway release of sequestered calcium ion into cytosol positive regulation of filopodium assembly positive regulation of protein kinase B signaling positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus response to nitric oxide interleukin-12 secretion positive regulation of neutrophil chemotaxis activation of GTPase activity lymphocyte migration into lymph node mature conventional dendritic cell differentiation negative regulation of leukocyte apoptotic process positive regulation of cell motility positive regulation of dendritic cell chemotaxis positive regulation of immunological synapse formation positive regulation of T cell costimulation positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation regulation of dendritic cell dendrite assembly
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