Search

  • Auftragsstatus
  • Eilbestellung
  • Aktionen

Invitrogen

CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet™ 395, eBioscience™

View all (66) CCR7 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

363-1979-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (1.0 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet™ 395, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12

Conjugate

Brilliant Ultra Violet™ 395 Brilliant Ultra Violet™ 395 Brilliant Ultra Violet™ 395

Excitation/Emission Max

347/399 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2937088

Product Specific Information

Description
The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Tested
This 3D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 350 nm; Emission: 395 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; G-protein coupled receptor; CCR7; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor; unnamed protein product

View more View less

Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; EVI1

View more View less

UniProt ID: (Human) P32248

View more View less

Entrez Gene ID: (Human) 1236

View more View less

Function(s)
G-protein coupled receptor activity chemokine receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity
Process(es)
establishment of T cell polarity positive regulation of cell-matrix adhesion dendritic cell chemotaxis myeloid dendritic cell chemotaxis positive regulation of dendritic cell antigen processing and presentation positive regulation of hypersensitivity positive regulation of humoral immune response chemotaxis inflammatory response immune response signal transduction G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration calcium-mediated signaling positive regulation of actin filament polymerization positive regulation of pseudopodium assembly ruffle organization regulation of Cdc42 protein signal transduction response to lipopolysaccharide regulation of interferon-gamma production regulation of interleukin-1 beta production negative regulation of interleukin-12 production positive regulation of interleukin-12 production positive regulation of Rac protein signal transduction CCL19-activated CCR7 signaling pathway CCL21-activated CCR7 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling negative thymic T cell selection positive regulation of cell adhesion positive regulation of JNK cascade positive regulation of receptor-mediated endocytosis homeostasis of number of cells positive regulation of T cell receptor signaling pathway release of sequestered calcium ion into cytosol positive regulation of filopodium assembly positive regulation of protein kinase B signaling cell chemotaxis positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus cellular response to prostaglandin E stimulus response to nitric oxide positive regulation of neutrophil chemotaxis lymphocyte migration into lymph node mature conventional dendritic cell differentiation positive regulation of phospholipase C/protein kinase C signal transduction positive regulation of cell motility positive regulation of dendritic cell chemotaxis positive regulation of immunological synapse formation positive regulation of T cell costimulation positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation regulation of dendritic cell dendrite assembly negative regulation of dendritic cell apoptotic process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support