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IFNAR1 Monoclonal Antibody (MAR1-5A3), PE, eBioscience™
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FIGURE: 1 / 1
IFNAR1 Antibody (12-5945-80) in Flow
Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.06 µg of Anti-Mouse IFN alpha/beta receptor I (IFNAR1) PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-5945-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MAR1-5A3
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.
Applications Reported: This MAR1-5A3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAR1-5A3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-& Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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