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Invitrogen
IFNAR1 Monoclonal Antibody (MAR1-5A3), Functional Grade, eBioscience™
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FIGURE: 1 / 5
IFNAR1 Antibody (16-5945-85) in Neu
PLoS pathogens 2024 -
10.1371/journal.ppat.1012179.g003 Fig 3 Differential macrophage replication of TC83 and TC83/E1 ID-syn viruses is IFN- and RLR-independent. (A-C) Replication kinetics of VEEV TC83 and TC83/E1 ID-syn in (A) empty vector, (B) Ddx58 -/- , and (C) Ifih1 -/- CRISPR Raw264.7 cells. Cells were infected with indicated viruses at a MOI of 0.1. Cell culture supernatant was serially harvested at 1, 6, 12, 24, 36, and ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-5945-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Bacteria,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
MAR1-5A3
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.
Applications Reported:The MAR1-5A3 antibody has been reported for use in neutralization of IFNAR1 bioactivity.
Applications Tested: The Functional Grade Purified MAR1-5A3 antibody has been tested by LAL assay to verify low endotoxin levels, by bioassay for neutralization of mouse IFNAR-1 bioactivity. The MAR1-5A3 antibody at 0.04 µg/mL has been found to inhibit by 50% the biological effects of 0.5 ng/mL mouse IFN-a2 (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in BestProtocols® section.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-& Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H3K1; IFN R1; IFN-alpha/beta receptor 1; IFN-alpha/betaR; IFN-alpha/betaR1; IFN-alpha/betaRalpha; IFN-R-1; IFNalpha/beta R1; IFNalpha/beta-Ralpha; IFNalpha/betaR1; IFNR1; INF-a receptor; type I interferon receptor 1; unnamed protein product
Gene Aliases: Ifar; Ifnar; Ifrc; Infar
UniProt ID: (Mouse) P33896
Entrez Gene ID: (Mouse) 15975
regulation of transcription from RNA polymerase II promoter
JAK-STAT cascade
response to virus
gene expression
cytokine-mediated signaling pathway
response to lipopolysaccharide
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 beta production
cellular response to interferon-alpha
cellular response to interferon-beta
T cell activation
positive regulation of transcription, DNA-templated
regulation of JAK-STAT cascade
regulation of peptidyl-tyrosine phosphorylation
defense response to virus
type I interferon signaling pathway
positive regulation of protein localization to nucleus
positive regulation of cellular respiration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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