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Invitrogen
IL-17A Monoclonal Antibody (eBio64DEC17), PE, eBioscience™
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FIGURE: 1 / 18
IL-17A Antibody (12-7179-42) in Flow
CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 6 hours. Cells were intracellular stained with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human IL-17A FITC using the Fixation & Permeabilization Buffers (Product # 88-8824-00). Viable cel... View More
Product Details
12-7179-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Dog,
Hamster,
Human,
Mouse,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio64DEC17
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies.
Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; inflammatory cytokine; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A; ILA17
UniProt ID: (Human) Q16552
Entrez Gene ID: (Human) 3605
positive regulation of antimicrobial peptide production
adaptive immune response
immune system process
apoptotic process
inflammatory response
immune response
Notch signaling pathway
cell-cell signaling
cell death
response to wounding
gene expression
positive regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-16 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
interleukin-17A-mediated signaling pathway
keratinocyte proliferation
innate immune response
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive regulation of protein kinase B signaling
intestinal epithelial structure maintenance
cellular response to interleukin-1
fibroblast activation
regulation of primary metabolic process
interleukin-17-mediated signaling pathway
granulocyte migration
negative regulation of inflammatory response to wounding
positive regulation of cytokine production involved in inflammatory response
positive regulation of bicellular tight junction assembly
positive regulation of tumor necrosis factor superfamily cytokine production
negative regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-X-C motif) ligand 1 production
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