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IL-37 Monoclonal Antibody (37D12), PE, eBioscience™
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FIGURE: 1 / 2
IL-37 Antibody (12-7379-42) in Flow
Normal human peripheral blood cells were stimulated overnight with Lipopolysaccharide (LPS) Solution (Product # 00-4976-03) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03). The cells were stained with Anti-Human CD14 APC (Product # 17-0149-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human IL-37 (IL-1F7) PE (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.
Product Details
12-7379-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
37D12
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 37D12 monoclonal antibody reacts with human IL-37. IL-37 is a member of the interleukin 1 cytokine family and is also known as IL-1F7 and IL-1H4. IL-37 suppresses innate inflammatory and immune responses and exhibits anti-inflammatory characteristics resembling those of IL-1R8. IL-37 binds to the alpha chain of IL-18R with low affinity and does not recruit the essential signaling co-receptor IL-18 beta or mediate pro-inflammatory activity. IL-37 also binds to IL-18 binding protein (IL-18BP) and enhances the antagonistic effects of IL-18BP. IL-37 forms a trimeric complex with IL-18BP and IL-18R beta, which results in the blockade of IL-18 activity. Transcripts of IL-37 have been detected in human tissues, such as lung, testis, and colon tumors, as well as some human cell lines.
Applications Reported: This 37D12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 37D12 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; alternatively spliced; cytokine; hIL-1H4; FIL1 zeta; IL-1 zeta; IL-1F7; IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1H; IL-1RP1; IL-1X; IL-1X protein; IL-37; IL1F7 (canonical product IL-1F7b); ILN; Interleukin; interleukin 1 family member 7; interleukin 1, zeta; Interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein; interleukin-23; Interleukin-37; Interleukin37; similar to IL-1
Gene Aliases: FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H; IL-1H4; IL-1RP1; IL-23; IL-37; IL1F7; IL1H4; IL1RP1; IL37
UniProt ID: (Human) Q9NZH6
Entrez Gene ID: (Human) 27178
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