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Invitrogen
IL-37 Monoclonal Antibody (37D12), eBioscience™
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FIGURE: 1 / 3
IL-37 Antibody (14-7379-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized human PBMC (stimulated with LPS and monensin for 5 hours) stained with 20 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 20 µg/mL of Anti-Human IL-37 (IL-1F7) Purified.Cells were then stained with F (ab')2 Anti-Mouse IgG eFluor® 570 followed by cytospin.Nuclei are stained with DAPI.
Product Details
14-7379-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
37D12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 37D12 monoclonal antibody reacts with human IL-37. IL-37 is a member of the interleukin 1 cytokine family and is also known as IL-1F7 and IL-1H4. IL-37 suppresses innate inflammatory and immune responses and exhibits anti-inflammatory characteristics resembling those of IL-1R8. IL-37 binds to the alpha chain of IL-18R with low affinity and does not recruit the essential signaling co-receptor IL-18 beta or mediate pro-inflammatory activity. IL-37 also binds to IL-18 binding protein (IL-18BP) and enhances the antagonistic effects of IL-18BP. IL-37 forms a trimeric complex with IL-18BP and IL-18R beta, which results in the blockade of IL-18 activity. Transcripts of IL-37 have been detected in human tissues, such as lung, testis, and colon tumors, as well as some human cell lines.
Applications Reported: This 37D12 antibody has been reported for use in immunocytochemistry followed by cytospin.
Applications Tested: This 37D12 antibody has been tested by immunocytochemistry of fixed and permeabilized normal human peripheral blood mononuclear cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine; alternatively spliced; cytokine; hIL-1H4; FIL1 zeta; IL-1 zeta; IL-1F7; IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1H; IL-1RP1; IL-1X; IL-1X protein; IL-37; IL1F7 (canonical product IL-1F7b); ILN; Interleukin; interleukin 1 family member 7; interleukin 1, zeta; Interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein; interleukin-23; Interleukin-37; Interleukin37; similar to IL-1
Gene Aliases: FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H; IL-1H4; IL-1RP1; IL-23; IL-37; IL1F7; IL1H4; IL1RP1; IL37
UniProt ID: (Human) Q9NZH6
Entrez Gene ID: (Human) 27178
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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