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CD230 (PrP) Monoclonal Antibody (4D5), PE, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-9230-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
4D5
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation.
Applications Reported: This 4D5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4D5 antibody has been pre-titrated and tested. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BSE; CD230 antigen; MGC26679; prion protein; prion-related protein; PRNP; PrP; TSE; unnamed protein product
Gene Aliases: AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
UniProt ID: (Human) F7VJQ1
Entrez Gene ID: (Human) 5621
beta-amyloid binding
protease binding
copper ion binding
protein binding
lamin binding
glycosaminoglycan binding
microtubule binding
tubulin binding
aspartic-type endopeptidase inhibitor activity
type 5 metabotropic glutamate receptor binding
type 8 metabotropic glutamate receptor binding
signaling receptor activity
identical protein binding
ATP-dependent protein binding
ion channel binding
macromolecular complex binding
metal ion binding
chaperone binding
binding, bridging
molecular function activator activity
molecular condensate scaffold activity
cupric ion binding
cuprous ion binding
negative regulation of transcription from RNA polymerase II promoter
cellular copper ion homeostasis
response to oxidative stress
learning or memory
long-term memory
negative regulation of protein processing
protein destabilization
negative regulation of interferon-gamma production
negative regulation of interleukin-17 production
negative regulation of interleukin-2 production
intracellular signal transduction
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of activated T cell proliferation
response to cadmium ion
response to copper ion
positive regulation of calcium-mediated signaling
negative regulation of T cell receptor signaling pathway
protein homooligomerization
negative regulation of calcineurin-NFAT signaling cascade
cellular response to copper ion
positive regulation of protein targeting to membrane
dendritic spine maintenance
negative regulation of long-term synaptic potentiation
regulation of glutamate receptor signaling pathway
positive regulation of glutamate receptor signaling pathway
negative regulation of beta-amyloid formation
negative regulation of dendritic spine maintenance
negative regulation of amyloid precursor protein catabolic process
positive regulation of protein localization to plasma membrane
response to amyloid-beta
cellular response to beta-amyloid
regulation of calcium ion import across plasma membrane
neuron projection maintenance
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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