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Invitrogen

CD230 (PrP) Monoclonal Antibody (4D5), eBioscience™

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4 Published Figures
4 References
View all (35) PrP antibodies

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Product Details

14-9230-82

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test
View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4D5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837141

Product Specific Information

Description: CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation.

Applications Reported: This 4D5 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The 4D5 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 35 kD under non-reducing conditions.

Applications Tested: The 4D5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alternative prion protein; AltPrP; ASCR; BSE; CD230; CD230 antigen; Major prion protein; MGC26679; prion protein; prion-related protein; PRNP; PrP; PrP27-30; PrP33-35C; TSE; unnamed protein product

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Gene Aliases: ALTPRP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PRNP; PRP; PrP27-30; PrP33-35C; PrPc

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UniProt ID: (Human) P04156

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Entrez Gene ID: (Human) 5621

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Function(s)
beta-amyloid binding protease binding copper ion binding protein binding lamin binding glycosaminoglycan binding microtubule binding tubulin binding aspartic-type endopeptidase inhibitor activity type 5 metabotropic glutamate receptor binding type 8 metabotropic glutamate receptor binding signaling receptor activity identical protein binding ATP-dependent protein binding ion channel binding macromolecular complex binding metal ion binding chaperone binding binding, bridging molecular function activator activity molecular condensate scaffold activity cupric ion binding cuprous ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cellular copper ion homeostasis response to oxidative stress learning or memory long-term memory negative regulation of protein processing protein destabilization negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-2 production regulation of protein localization intracellular signal transduction negative regulation of apoptotic process positive regulation of neuron apoptotic process negative regulation of activated T cell proliferation response to cadmium ion response to copper ion positive regulation of calcium-mediated signaling negative regulation of T cell receptor signaling pathway protein homooligomerization regulation of cell cycle negative regulation of calcineurin-NFAT signaling cascade cellular response to copper ion cellular response to xenobiotic stimulus positive regulation of protein targeting to membrane dendritic spine maintenance negative regulation of long-term synaptic potentiation regulation of glutamate receptor signaling pathway positive regulation of glutamate receptor signaling pathway regulation of potassium ion transmembrane transport negative regulation of beta-amyloid formation negative regulation of dendritic spine maintenance negative regulation of amyloid precursor protein catabolic process positive regulation of protein localization to plasma membrane response to amyloid-beta cellular response to beta-amyloid regulation of calcium ion import across plasma membrane neuron projection maintenance
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