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CD32 Monoclonal Antibody (6C4 (CD32)), Biotin, eBioscience™
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FIGURE: 1 / 3
CD32 Antibody (13-0329-82) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control, Biotin (Product # 13-4714-85) (left) or CD32 Monoclonal Antibody, Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-0329-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
6C4 (CD32)
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.
Applications Reported: This 6C4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 6C4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD32 receptor 2; CD32 receptor 2a; CDw32; Fc fragment of IgG receptor IIa; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIc; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor IIC; Fc gamma receptor RIIa3; Fc gamma RIIb; fc-gamma RII-b; fc-gamma-RIIa; fc-gamma-RIIb; fc-gamma-RIIc; FCGR2B; FcgRIIa3 splice variant; fcRII-a; fcRII-b; FGFR2B; IgG Fc fragment receptor; IgG Fc fragment receptor precursor; IgG Fc receptor alpha-Fc-gamma-RII; IgG Fc receptor beta-Fc-gamma-RII; igG Fc receptor II-a; igG Fc receptor II-b; IgG Fc receptor II-c; IgG Fc receptor precursor; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; MGC23887; MGC30032; RP11-5K23.6; unnamed protein product
Gene Aliases: CD32; CD32A; CD32B; CD32C; CDW32; FCG2; FcgammaRIIa; FcgammaRIIc; FcGR; FCGR2; FCGR2A1; FCRIIC; IGFR2
UniProt ID: (Human) P12318, (Human) P31994, (Human) P31995
Entrez Gene ID: (Human) 2212, (Human) 2213, (Human) 9103
antibody-dependent cellular cytotoxicity
regulation of immune system process
immune response
cell surface receptor signaling pathway
positive regulation of tumor necrosis factor production
Fc-gamma receptor signaling pathway
positive regulation of phagocytosis
negative regulation of type I hypersensitivity
negative regulation of antibody-dependent cellular cytotoxicity
negative regulation of cytokine production
immune effector process
follicular dendritic cell activation
mature B cell differentiation involved in immune response
follicular B cell differentiation
immune complex clearance by monocytes and macrophages
negative regulation of dendritic cell antigen processing and presentation
regulation of B cell antigen processing and presentation
negative regulation of immunoglobulin production
regulation of adaptive immune response
negative regulation of acute inflammatory response to antigenic stimulus
positive regulation of humoral immune response
negative regulation of humoral immune response mediated by circulating immunoglobulin
receptor-mediated endocytosis
phagocytosis
defense response
inflammatory response
response to bacterium
immunoglobulin mediated immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of B cell proliferation
negative regulation of interleukin-10 production
negative regulation of macrophage activation
negative regulation of cytotoxic T cell degranulation
regulation of innate immune response
positive regulation of JNK cascade
negative regulation of phagocytosis
negative regulation of immune response
negative regulation of B cell receptor signaling pathway
negative regulation of B cell activation
regulation of immune complex clearance by monocytes and macrophages
negative regulation of neutrophil activation
regulation of dendritic spine maintenance
regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway
cellular response to beta-amyloid
positive regulation of response to endoplasmic reticulum stress
negative regulation of dendritic cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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