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Invitrogen

CD32 Monoclonal Antibody (6C4 (CD32)), PE-Cyanine5.5, eBioscience™

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Product Details

35-0329-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C4 (CD32)

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811799

Product Specific Information

Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.

Applications Reported: This 6C4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6C4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: CD32 receptor 2; CD32 receptor 2a; CDw32; Fc fragment of IgG receptor IIa; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIc; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor IIC; Fc gamma receptor RIIa3; Fc gamma RIIb; fc-gamma RII-b; fc-gamma-RIIa; fc-gamma-RIIb; fc-gamma-RIIc; FCGR2B; FcgRIIa3 splice variant; fcRII-a; fcRII-b; FGFR2B; IgG Fc fragment receptor; IgG Fc fragment receptor precursor; IgG Fc receptor alpha-Fc-gamma-RII; IgG Fc receptor beta-Fc-gamma-RII; igG Fc receptor II-a; igG Fc receptor II-b; IgG Fc receptor II-c; IgG Fc receptor precursor; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; MGC23887; MGC30032; RP11-5K23.6; unnamed protein product

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Gene Aliases: CD32; CD32A; CD32B; CD32C; CDW32; FCG2; FcgammaRIIa; FcgammaRIIc; FcGR; FCGR2; FCGR2A1; FCRIIC; IGFR2

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UniProt ID: (Human) P12318, (Human) P31994, (Human) P31995

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Entrez Gene ID: (Human) 2212, (Human) 2213, (Human) 9103

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Function(s)
protein binding IgG receptor activity IgG binding beta-amyloid binding low-affinity IgG receptor activity macromolecular complex binding transmembrane signaling receptor activity
Process(es)
antibody-dependent cellular cytotoxicity regulation of immune system process immune response cell surface receptor signaling pathway positive regulation of tumor necrosis factor production Fc-gamma receptor signaling pathway positive regulation of phagocytosis negative regulation of type I hypersensitivity negative regulation of antibody-dependent cellular cytotoxicity negative regulation of cytokine production immune effector process follicular dendritic cell activation mature B cell differentiation involved in immune response follicular B cell differentiation immune complex clearance by monocytes and macrophages negative regulation of dendritic cell antigen processing and presentation regulation of B cell antigen processing and presentation negative regulation of immunoglobulin production regulation of adaptive immune response negative regulation of acute inflammatory response to antigenic stimulus positive regulation of humoral immune response negative regulation of humoral immune response mediated by circulating immunoglobulin receptor-mediated endocytosis phagocytosis defense response inflammatory response response to bacterium immunoglobulin mediated immune response antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of B cell proliferation negative regulation of interleukin-10 production negative regulation of macrophage activation negative regulation of cytotoxic T cell degranulation regulation of innate immune response positive regulation of JNK cascade negative regulation of phagocytosis negative regulation of immune response negative regulation of B cell receptor signaling pathway negative regulation of B cell activation regulation of immune complex clearance by monocytes and macrophages negative regulation of neutrophil activation regulation of dendritic spine maintenance regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway cellular response to beta-amyloid positive regulation of response to endoplasmic reticulum stress negative regulation of dendritic cell differentiation
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