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CD39 Monoclonal Antibody (eBioA1 (A1)), eBioscience™
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FIGURE: 1 / 20
CD39 Antibody (14-0399-82) in IHC (P)
Immunohistochemical analysis of CD39 was performed using formalin-fixed paraffin-embedded human thyroid (papillary carcinoma) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsiu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-0399-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioA1 (A1)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.
Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.
Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.
Applications Tested: This eBioA1 (A1) antibody has been tested by flow cytometric analysis of nomral human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD39 is an integral membrane protein characterized by two transmembrane domains and forms a homotetramer structure. It is recognized as the most prominent ectoenzyme within the immune system, primarily functioning to remove toxic extracellular ATP by converting it into ADP or AMP. CD39 works in conjunction with CD73 to hydrolyze ATP and is well characterized on Langerhans cells. Originally identified on activated lymphocytes, CD39 is expressed on a variety of immune cells, including peripheral blood B cells, monocytes, and T cell clones. It is also found on a subset of T cells, B cells, and dendritic cells, with weaker expression observed on monocytes and granulocytes. The CD39 cell surface antigen is a 70-100kD molecule, and its expression is induced on T cells and increases on B cells as a late activation antigen. CD39 possesses intrinsic ecto-ATPase activity, playing a crucial role in modulating extracellular nucleotide levels and contributing to immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP diphosphohydrolase; ATPDase; CD39 antigen; DKFZp686D194; DKFZp686I093; ecto-apyrase; ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; ecto-ATPDase 1; ectoapyrase; FLJ40921; FLJ40959; guanosine diphosphatase homolog; lymphoid cell activation antigen; NTPDase 1; NTPDase1; nucleoside triphosphate diphosphohydrolase 1; unnamed protein product
Gene Aliases: 2610206B08Rik; ATP-DPH; ATPDase; CD39; E130009M23Rik; NTPDase-1; SPG64
UniProt ID: (Human) P49961, (Mouse) P55772
Entrez Gene ID: (Human) 953, (Mouse) 12495
GTPase activity
obsolete apyrase activity
guanosine-diphosphatase activity
protein binding
pyrophosphatase activity
hydrolase activity
ATPase activity
nucleoside-diphosphatase activity
nucleoside-triphosphatase activity
cytidine diphosphatase activity
adenosine-diphosphatase activity
CTPase activity
uridine-diphosphatase activity
ITPase activity
IDP phosphatase activity
identical protein binding
G-protein coupled receptor signaling pathway
nucleoside diphosphate catabolic process
purine ribonucleoside diphosphate catabolic process
ribonucleoside diphosphate catabolic process
platelet activation
negative regulation of dopamine secretion
ADP catabolic process
platelet aggregation
negative regulation of ATP biosynthetic process
cell adhesion
blood coagulation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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