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Invitrogen
CD39 Monoclonal Antibody (eBioA1 (A1)), PE, eBioscience™
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FIGURE: 1 / 16
CD39 Antibody (12-0399-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD39 PE, followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00), and intracellular staining with Anti-Human Foxp3 APC (Product # 17-4776-42). Cells in the lymphocyte gate were used for analysis.
Product Details
12-0399-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioA1 (A1)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.
Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.
Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioA1 (A1) antibody has been pre-titrated and tested flow cytometric analysis of normal human peripheral blood cells. his can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD39 is an integral membrane protein characterized by two transmembrane domains and forms a homotetramer structure. It is recognized as the most prominent ectoenzyme within the immune system, primarily functioning to remove toxic extracellular ATP by converting it into ADP or AMP. CD39 works in conjunction with CD73 to hydrolyze ATP and is well characterized on Langerhans cells. Originally identified on activated lymphocytes, CD39 is expressed on a variety of immune cells, including peripheral blood B cells, monocytes, and T cell clones. It is also found on a subset of T cells, B cells, and dendritic cells, with weaker expression observed on monocytes and granulocytes. The CD39 cell surface antigen is a 70-100kD molecule, and its expression is induced on T cells and increases on B cells as a late activation antigen. CD39 possesses intrinsic ecto-ATPase activity, playing a crucial role in modulating extracellular nucleotide levels and contributing to immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; guanosine diphosphatase homolog; Lymphoid cell activation antigen; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1; unnamed protein product
Gene Aliases: ATP-DPH; ATPDase; CD39; ENTPD1; NTPDase-1; SPG64
UniProt ID: (Human) P49961
Entrez Gene ID: (Human) 953
nucleotide binding
GTPase activity
obsolete apyrase activity
guanosine-diphosphatase activity
protein binding
ATP binding
hydrolase activity
ATPase activity
nucleoside-diphosphatase activity
nucleoside-triphosphatase activity
cytidine diphosphatase activity
adenosine-diphosphatase activity
CTPase activity
uridine-diphosphatase activity
ITPase activity
IDP phosphatase activity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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