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Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), eBioscience™
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FIGURE: 1 / 50
Endomucin Antibody (14-5851-82) in WB
Western blot was performed using Anti-Endomucin Monoclonal Antibody (Product # 14-5851-85) and an 80kDa band corresponding to Endomucin was observed across tissue lysates tested except Mouse Bone and Mouse Adipose. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Mouse Lung (Lane 2), Mouse Bone (Lane 3) and Mouse Adipose (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting Syst... View More
Product Details
14-5851-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioV.7C7 (V.7C7)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, which was identified in a search for cell-surface expressed endothelial cell markers. Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however, an exception is the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.
Applications Reported: This eBioV.7C7 (V.7C7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This eBioV.7C7 (V.7C7) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Endomucin (EMCN) also known as endothelial sialomucin or mucin-like sialoglycoprotein interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endomucin; endomucin-1; Endomucin-1/2; MUC-14; mucin 14, endothelial; Mucin-14
Gene Aliases: 0610012K22Rik; AI315669; Emcn; Muc14
UniProt ID: (Mouse) Q9R0H2
Entrez Gene ID: (Mouse) 59308
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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