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Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 22
Endomucin Antibody (53-5851-82) in Flow
bEnd3 cells were stained with 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (blue histogram) or 0.5 µg of Endomucin Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
53-5851-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioV.7C7 (V.7C7)
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, which was identified in a search for cell-surface expressed endothelial cell markers. Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however, an exception is the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.
Applications Reported: This eBioV.7C7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioV.7C7 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
Endomucin (EMCN) also known as endothelial sialomucin or mucin-like sialoglycoprotein interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endomucin; endomucin-1; Endomucin-1/2; MUC-14; mucin 14, endothelial; Mucin-14
Gene Aliases: 0610012K22Rik; AI315669; Emcn; Muc14
UniProt ID: (Mouse) Q9R0H2
Entrez Gene ID: (Mouse) 59308
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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