Chorionic Gonadotropin Monoclonal Antibody (FB12), eBioscience™

Product Specific Information

Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal).

Applications Reported: This FB12 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections (IHC-F), ELISA, and immunocytochemistry (ICC).

Applications Tested: This FB12 antibody has been tested by western blot and immunocytochemistry on BeWo cells. This can be used at less than or equal to 5 µg/mL. it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.