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Invitrogen
Chorionic Gonadotropin Monoclonal Antibody (FB12), Alexa Fluor™ 488, eBioscience™
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Product Details
53-6508-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FB12
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal).
Applications Reported: This FB12 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F) and immunocytochemistry (ICC).
Applications Tested: This FB12 antibody has been tested by immunocytochemistry on fixed BeWo cells. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Anterior pituitary glycoprotein hormones common subunit alpha; CG-alpha; CG-beta; CGA; Choriogonadotropin alpha chain; Choriogonadotropin subunit beta; Choriogonadotropin subunit beta 3; chorionic gonadotrophin chain beta; Chorionic gonadotrophin subunit alpha; chorionic gonadotropin beta 3 subunit; chorionic gonadotropin beta 5 subunit; chorionic gonadotropin beta 8 subunit; chorionic gonadotropin beta chain; chorionic gonadotropin beta subunit 3; chorionic gonadotropin beta subunit 5; chorionic gonadotropin beta subunit 8; Chorionic gonadotropin chain beta; chorionic gonadotropin human; chorionic gonadotropin, alpha polypeptide; chorionic gonadotropin, beta polypeptide; Follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; Follitropin alpha chain; FSH-alpha; Glycoprotein hormones alpha chain; human chorionic gonadotropin; LSH-alpha; Luteinizing hormone alpha chain; luteinizing hormone beta subunit; Lutropin alpha chain; Thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSH-alpha; unnamed protein product
Gene Aliases: CG-ALPHA; CGA; CGB; CGB3; CGB5; CGB7; CGB8; FSHA; GPA1; GPHa; GPHA1; HCG; hCGB; LHA; LHB; TSHA
UniProt ID: (Human) P01215, (Human) P0DN86
Entrez Gene ID: (Human) 1081, (Human) 1082, (Human) 93659, (Human) 94115
apoptotic process
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
female gamete generation
hormone-mediated signaling pathway
thyroid hormone generation
positive regulation of cell proliferation
gonad development
regulation of receptor activity
positive regulation of steroid biosynthetic process
positive regulation of cell migration
thyroid gland development
luteinizing hormone secretion
cellular response to hormone stimulus
organ growth
follicle-stimulating hormone signaling pathway
positive regulation of transcription from RNA polymerase II promoter
negative regulation of organ growth
follicle-stimulating hormone secretion
developmental growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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