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Invitrogen
CD282 (TLR2) Monoclonal Antibody (6C2), eBioscience™
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FIGURE: 1 / 17
CD282 (TLR2) Antibody (14-9021-82) in ICC/IF
Immunofluorescence analysis of TLR2 was performed using Raw 264.7 cells and Raw 264.7 treated with LPS (500 ng/mL, 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TLR2 Rat Monoclonal Antibody (Product # 14-9021-82) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat a... View More
Product Details
14-9021-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
6C2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.
Applications Reported: This 6C2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, Product # 14-0161).
Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes or Raw264.7 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD282; TLR-2; Toll-like receptor 2
Gene Aliases: Ly105; Tlr2
UniProt ID: (Mouse) Q9QUN7
Entrez Gene ID: (Mouse) 24088
microglial cell activation
toll-like receptor signaling pathway
response to molecule of bacterial origin
response to molecule of fungal origin
cytokine secretion involved in immune response
immune system process
positive regulation of leukocyte migration
regulation of dendritic cell cytokine production
cell surface pattern recognition receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
leukotriene metabolic process
inflammatory response
immune response
signal transduction
I-kappaB phosphorylation
negative regulation of cell proliferation
response to bacterium
positive regulation of Wnt signaling pathway
central nervous system myelin formation
response to bacterial lipoprotein
response to peptidoglycan
interleukin-10 production
negative regulation of interleukin-12 production
negative regulation of interleukin-17 production
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 2 signaling pathway
positive regulation of NF-kappaB import into nucleus
detection of triacyl bacterial lipopeptide
detection of diacyl bacterial lipopeptide
positive regulation of tumor necrosis factor biosynthetic process
chloramphenicol transport
negative regulation of growth of symbiont in host
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
nitric oxide metabolic process
positive regulation of oligodendrocyte differentiation
regulation of cytokine secretion
positive regulation of cytokine secretion
positive regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
induction by symbiont of defense-related host nitric oxide production
positive regulation of macrophage cytokine production
response to fatty acid
cellular response to bacterial lipopeptide
cellular response to lipoteichoic acid
cellular response to peptidoglycan
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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