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Invitrogen

CD282 (TLR2) Monoclonal Antibody (6C2), Functional Grade, eBioscience™

17 Published Figures
26 References
View all (75) TLR2 antibodies

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Product Details

16-9021-81

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test
View 13 publications 13 publications

Neutralization (Neu)

-
View 4 publications 4 publications

Functional Assay (Functional)

Assay-Dependent
View 2 publications 2 publications

Control (Ctrl)

Assay-Dependent
-

Inhibition Assays (Inhibition)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C2

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469265

Product Specific Information

Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported: 6C2 has been reported for use in flow cytometric analysis. Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, Product # 14-0161).

Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes or Raw264.7 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2

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Gene Aliases: Ly105; Tlr2

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UniProt ID: (Mouse) Q9QUN7

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Entrez Gene ID: (Mouse) 24088

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Function(s)
receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity triacyl lipopeptide binding diacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity lipoteichoic acid binding lipopeptide binding
Process(es)
microglial cell activation toll-like receptor signaling pathway response to molecule of bacterial origin response to molecule of fungal origin cytokine secretion involved in immune response immune system process positive regulation of leukocyte migration regulation of dendritic cell cytokine production cell surface pattern recognition receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway leukotriene metabolic process inflammatory response immune response signal transduction I-kappaB phosphorylation negative regulation of cell proliferation response to bacterium positive regulation of Wnt signaling pathway central nervous system myelin formation response to bacterial lipoprotein response to peptidoglycan interleukin-10 production negative regulation of interleukin-12 production negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide positive regulation of tumor necrosis factor biosynthetic process chloramphenicol transport negative regulation of growth of symbiont in host innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of oligodendrocyte differentiation regulation of cytokine secretion positive regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process induction by symbiont of defense-related host nitric oxide production positive regulation of macrophage cytokine production response to fatty acid cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide
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