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Invitrogen

HSP70 Monoclonal Antibody (1B2hsp70), eBioscience™

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Product Details

14-9115-37

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
-
Product Specifications

Species Reactivity

Dog, Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1B2hsp70

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

2 mg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865351

Product Specific Information

Description: The monoclonal antibody 1B2hsp70 recognizes HSP (heat shock protein) 70. Hsps are highly conserved molecular chaperones that associate with proteins to stabilize or aid in the folding of new proteins or denatured proteins following chemical or physical stress. Hsp expression is both constitutive and inducible depending on the cell and its surrounding conditions. Hsps have been known to be upregulated in conditions such as cancer, ischemic injury, inflammatory, and demyelinating disease.

This antibody recognizes the Hsp70 family member 1A found in the cytoplasm and nucleus of numerous cell types. Given the high degree of conservation of this protein across species, the 1B2hsp70 clone is expected to recognize canine and monkey HSP70 1a.

Applications Reported: This 1B2hsp70 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This 1B2hsp70 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using both low and high pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 70 kilodalton heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; Heat shock protein; heat shock protein Apg-2; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock-induced protein; Heat shock-related 70 kDa protein 2; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1/HSP70.2; HSP70RY; HSPA2; HSPA4; Hspa70; unnamed protein product

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Gene Aliases: APG-2; APG2; HEL-S-103; HEL-S-5a; HS24/P52; HSP70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; HSP70I; hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; HSX70; hum70t; RY

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UniProt ID: (Dog) Q7YQC6, (Human) P0DMV8, (Human) P34931, (Human) P54652, (Dog) Q2TFN9, (Human) P34932

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Entrez Gene ID: (Dog) 403612, (Human) 3303, (Human) 3304, (Human) 3305, (Dog) 480355, (Human) 3306, (Dog) 474680, (Human) 3308

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Function(s)
nucleotide binding virus receptor activity G-protein coupled receptor binding transcription corepressor activity RNA binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding denatured protein binding ubiquitin protein ligase binding death receptor agonist activity histone deacetylase binding protein binding involved in protein folding cadherin binding receptor agonist activity unfolded protein binding misfolded protein binding C3HC4-type RING finger domain binding disordered domain specific binding transcription regulator inhibitor activity ATP-dependent protein disaggregase activity ATP-dependent protein folding chaperone tau protein binding chaperone binding glycolipid binding adenyl-nucleotide exchange factor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter mRNA catabolic process protein folding response to unfolded protein lysosomal transport negative regulation of cell proliferation positive regulation of gene expression negative regulation of cell growth negative regulation of transforming growth factor beta receptor signaling pathway endoplasmic reticulum unfolded protein response regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of interleukin-8 production positive regulation of RNA splicing cellular response to oxidative stress cellular response to heat cellular response to unfolded protein protein refolding negative regulation of apoptotic process positive regulation of erythrocyte differentiation ATP metabolic process viral entry into host cell protein stabilization positive regulation of NF-kappaB transcription factor activity chaperone-mediated protein complex assembly cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to steroid hormone stimulus positive regulation of microtubule nucleation negative regulation of inclusion body assembly negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitotic spindle assembly negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of tumor necrosis factor-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand male meiosis male meiosis I spermatogenesis spermatid development response to heat response to cold positive regulation of G2/M transition of mitotic cell cycle cell differentiation synaptonemal complex disassembly response to stress binding of sperm to zona pellucida positive regulation of protein targeting to mitochondrion protein import into mitochondrial matrix
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