Search
Invitrogen
HSP70 Monoclonal Antibody (1B2hsp70), eFluor™ 660, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
50-9115-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1B2hsp70
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 1B2hsp70 recognizes HSP (heat shock protein) 70. Hsps are highly conserved molecular chaperones that associate with proteins to stabilize or aid in the folding of new proteins or denatured proteins following chemical or physical stress. Hsp expression is both constitutive and inducible depending on the cell and its surrounding conditions. Hsps have been known to be upregulated in conditions such as cancer, ischemic injury, inflammatory, and demyelinating disease.
This antibody recognizes the Hsp70 family member 1A found in the cytoplasm and nucleus of numerous cell types. Given the high degree of conservation of this protein across species, the 1B2hsp70 clone is expected to recognize canine and monkey HSP70 1a.
Applications Reported: This 1B2 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This 1B2 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 70 kilodalton heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; Heat shock protein; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock-induced protein; Heat shock-related 70 kDa protein 2; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1/HSP70.2; HSP70RY; HSPA2; HSPA4; Hspa70; unnamed protein product
Gene Aliases: APG-2; APG2; HEL-S-103; HEL-S-5a; HS24/P52; HSP70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; HSP70I; hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; HSX70; hum70t; RY
UniProt ID: (Human) P0DMV8, (Human) P34931, (Human) P54652, (Human) P34932
Entrez Gene ID: (Human) 3303, (Human) 3304, (Human) 3305, (Human) 3306, (Human) 3308
nucleotide binding
virus receptor activity
G-protein coupled receptor binding
transcription corepressor activity
RNA binding
receptor binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
denatured protein binding
ubiquitin protein ligase binding
death receptor agonist activity
histone deacetylase binding
protein binding involved in protein folding
cadherin binding
receptor agonist activity
unfolded protein binding
misfolded protein binding
C3HC4-type RING finger domain binding
disordered domain specific binding
transcription regulator inhibitor activity
ATP-dependent protein disaggregase activity
ATP-dependent protein folding chaperone
tau protein binding
chaperone binding
glycolipid binding
adenyl-nucleotide exchange factor activity
negative regulation of transcription from RNA polymerase II promoter
mRNA catabolic process
protein folding
response to unfolded protein
lysosomal transport
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of cell growth
negative regulation of transforming growth factor beta receptor signaling pathway
endoplasmic reticulum unfolded protein response
regulation of protein ubiquitination
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of interleukin-8 production
positive regulation of RNA splicing
cellular response to oxidative stress
cellular response to heat
cellular response to unfolded protein
protein refolding
negative regulation of apoptotic process
positive regulation of erythrocyte differentiation
ATP metabolic process
viral entry into host cell
protein stabilization
positive regulation of NF-kappaB transcription factor activity
chaperone-mediated protein complex assembly
cellular heat acclimation
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to steroid hormone stimulus
positive regulation of microtubule nucleation
negative regulation of inclusion body assembly
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of mitotic spindle assembly
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
male meiosis
male meiosis I
spermatogenesis
spermatid development
response to heat
response to cold
positive regulation of G2/M transition of mitotic cell cycle
cell differentiation
synaptonemal complex disassembly
response to stress
binding of sperm to zona pellucida
positive regulation of protein targeting to mitochondrion
protein import into mitochondrial matrix
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support