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Invitrogen
PU.1 Monoclonal Antibody (phpu13), eBioscience™
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FIGURE: 1 / 3
PU.1 Antibody (14-9819-80) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 10 µg/mL of mouse IgG1 isotype control (left) or 10 µg/mL Anti-Human/Mouse PU-1 Purified (right) followed by biotinylated anti-mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9819-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
phpu13
Immunogen
E. coli-derived protein, without DNA domain
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The monoclonal phpu13 recognizes human and mouse PU.1, a member of the ets family of transcription factors. This 42 kDa protein was originally identified as Spi-1, a proto-oncogene, for its role in Friend virus-induced murine erythroleukemia. Expression of PU.1 plays a critical role in lymphocyte and myeloid development. Knock-out mice are neonatal lethal and lack myeloid, T and B cells. In addition erythroid development is also altered. Protein expression levels help guarantee proper development; higher levels in myeloid cells compared to lymphocytes. PU.1 controls transcription of several proteins including CD11b, CD18, Gata2, IRF4, CD20, CD10, and CD79.
Disruption of PU.1 expression may play a role in several specific leukemias; such as B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin.
Applications Reported: This phpu13 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and immunocytochemistry.
Applications Tested: This phpu13 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58). This can be used at less than or equal to 10 µg/mL. It is recommeded that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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