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Invitrogen

PU.1 Monoclonal Antibody (phpu13), eBioscience™

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Product Details

14-9819-82

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

phpu13

Immunogen

E. coli-derived protein, without DNA domain

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151144

Product Specific Information

Description: The monoclonal phpu13 recognizes human and mouse PU.1, a member of the ets family of transcription factors. This 42 kDa protein was originally identified as Spi-1, a proto-oncogene, for its role in Friend virus-induced murine erythroleukemia. Expression of PU.1 plays a critical role in lymphocyte and myeloid development. Knock-out mice are neonatal lethal and lack myeloid, T and B cells. In addition erythroid development is also altered. Protein expression levels help guarantee proper development; higher levels in myeloid cells compared to lymphocytes. PU.1 controls transcription of several proteins including CD11b, CD18, Gata2, IRF4, CD20, CD10, and CD79.

Disruption of PU.1 expression may play a role in several specific leukemias; such as B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin.

Applications Reported: This phpu13 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and immunocytochemistry.

Applications Tested: This phpu13 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58). This can be used at less than or equal to 10 µg/mL. It is recommeded that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

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Gene Aliases: AGM10; Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1

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UniProt ID: (Human) P17947, (Mouse) P17433

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Entrez Gene ID: (Human) 6688, (Mouse) 20375

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding histone deacetylase binding sequence-specific DNA binding NFAT protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding STAT family protein binding DNA-binding transcription factor binding protein sequestering activity RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding winged helix/forkhead transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter vasculature development germinal center B cell differentiation follicular B cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation defense response to tumor cell pro-T cell differentiation myeloid leukocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway positive regulation of gene expression negative regulation of gene expression lymphocyte differentiation cell differentiation erythrocyte differentiation macrophage differentiation osteoclast differentiation granulocyte differentiation lipopolysaccharide-mediated signaling pathway somatic stem cell population maintenance TRAIL-activated apoptotic signaling pathway myeloid dendritic cell differentiation negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neutrophil degranulation negative regulation of MHC class II biosynthetic process response to ethanol positive regulation of B cell differentiation regulation of erythrocyte differentiation positive regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression interleukin-6-mediated signaling pathway cellular response to ethanol oncogene-induced cell senescence endothelial to hematopoietic transition negative regulation of protein localization to chromatin positive regulation of p38MAPK cascade negative regulation of NIK/NF-kappaB signaling apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of microglial cell mediated cytotoxicity negative regulation of adipose tissue development pericyte cell differentiation positive regulation of antifungal innate immune response regulation of myeloid progenitor cell differentiation positive regulation of myeloid dendritic cell chemotaxis transcription, DNA-templated transcription from RNA polymerase II promoter histone H3 acetylation hypermethylation of CpG island negative regulation of gene expression, epigenetic negative regulation of histone H4 acetylation
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