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Invitrogen
CD8b Monoclonal Antibody (SIDI8BEE), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 4
CD8b Antibody (25-5273-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0087-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human/Non-Human Primate CD8b PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
25-5273-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human,
Mouse,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SIDI8BEE
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta. The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells. Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well. SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer.
SIDI8BEE is cross-reactive to human and rhesus macaque CD8b. Other non-human primate species have not been tested.
Applications Reported: This SIDI8BEE antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SIDI8BEE antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD8; CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8b molecule; CD8beta; Leu-2; Ly-3 homolog; MAL; p32; T lymphocyte surface glycoprotein (CD8-beta) precursor; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain; unnamed protein product
Gene Aliases: CD8B; CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37
UniProt ID: (Human) P10966
Entrez Gene ID: (Human) 926
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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