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Invitrogen
TCR V beta F1 Monoclonal Antibody (8A3), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 2
TCR V beta F1 Antibody (25-5766-42) in Flow
Human PBMC were stained with CD3 Monoclonal Antibody, APC (Product # 17-0038-42) and TCR gamma/delta Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9959-42). The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and stained with Mouse IgG1 kappa Isotype Control, PE-Cyanine 7 (Product # 25-4714-42) (left) or TCR beta F1 Monoclonal Antibody, PE-Cyanine 7 (right). Viable CD3 positive lymphocytes were used for analysis.
Product Details
25-5766-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
8A3
Immunogen
Human TCR beta chain constant region
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determinant on the beta-subunit of the T cell receptor. Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS). Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia. This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells.
Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
The T Cell Receptor alpha beta (TCRab) is a heterodimeric membrane receptor that consists of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 proteins. TCRab participates in the activation of T-cells in response to an antigen presented in the context of MHC. Engagement of the TCRab initiates signaling cascades that ultimately result in cellular proliferation, differentiation, cytokine production, and/or activation-induced cell death. These signaling cascades regulate T-cell development, homeostasis, activation, acquisition of effector functions and apoptosis. Cells expressing TCRab constitute the vast majority of T cells in both human and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: tcr beta f1
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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