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Invitrogen

CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PE-Cyanine7, eBioscience™

View all (32) Dectin 1 (Clec7a) antibodies

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Product Details

25-5859-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

bg1fpj

Immunogen

Baculovirus-expressed Fc-containing protein

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573479

Product Specific Information

Description: The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family. The ligand is beta1, 3-glycan. Beta glycans are a main component in polysaccharides and specifically beta1,3-linked carbohydrates are found in the cell wall of fungi, plants and some bacteria. CD369 has been found to bind Candida, Aspergillis, Pneumocystis, Coccidioides and Mycobacterium. Calcium dependent binding to ligand signals through syk via the ITAM domain in the cytoplasmic portion and results in endocyotosis/phagocytosis and production of cytokines (IL-2, IL-6, IL-10, TNF alpha, IL-1 beta, IL-23). A direct role in Th17 and Th1 differentiation has been attributed to CD369 signaling. CD369 is expressed predominantly on myeloid cells (neutrophils, monocytes, macrophages, and dendritic cells). Low levels have also been reported on lymphocytes and mucosal epithelium.

Applications Reported: This bg1fpj antibody has been reported for use in flow cytometric analysis.

Applications Tested: This bg1fpj antibody has been tested by flow cytometric analysis of mouse lysed whole blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-glucan receptor; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type lectin superfamily member 12; DC-associated C-type lectin 1; dectin-1; dendritic cell-associated C-type lectin 1

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Gene Aliases: beta-GR; BGR; Clecsf12

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Entrez Gene ID: (Mouse) 56644

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Function(s)
opsonin binding (1->3)-beta-D-glucan binding (1->3)-beta-D-glucan receptor activity protein binding carbohydrate binding polysaccharide binding pattern recognition receptor activity identical protein binding
Process(es)
cell activation response to yeast detection of yeast stimulatory C-type lectin receptor signaling pathway response to molecule of fungal origin leukocyte activation involved in immune response positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production cell surface pattern recognition receptor signaling pathway phagocytosis, recognition phagocytosis, engulfment cell recognition positive regulation of cell proliferation positive regulation of gene expression detection of fungus positive regulation of cell migration positive regulation of protein complex assembly detection of molecule of fungal origin positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-2 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade positive regulation of superoxide anion generation regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade innate immune response leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of phagocytosis positive regulation of calcium-mediated signaling positive regulation of lymphocyte activation positive regulation of respiratory burst antifungal innate immune response regulation of calcineurin-NFAT signaling cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of fungal origin positive regulation of monocyte chemotactic protein-1 production positive regulation of wound healing apoptotic signaling pathway cell-cell adhesion positive regulation of cytokine production involved in inflammatory response positive regulation of cell maturation positive regulation of T-helper 17 type immune response
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