Search
Invitrogen
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), PE-Cyanine7, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 87
CD274 (PD-L1, B7-H1) Antibody (25-5983-42) in Flow
Staining of unstimulated (left) or PHA-stimulated (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD274 (B7-H1) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.
.jpg?time=20220826)
.jpg?time=20220826)
.jpg?time=20220826?time=20220826 "CD274 (PD-L1, B7-H1) Antibody (25-5983-42) in Flow")
.jpg?time=20220826?time=20220826 "CD274 (PD-L1, B7-H1) Antibody (25-5983-42) in Flow")
Product Details
25-5983-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH1
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: B7 homolog 1; B7-H1; B7-H1,PD-L1; CD274; CD274 antigen; costimulatory molecule; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death-1 ligand 1; sCD274; similar to Homo sapiens B7-1 and B7-2; soluble CD274; soluble PD L1; soluble PD-L1; B7-H1; sPD L1; unnamed protein product
Gene Aliases: B7-H; B7H1; CD274; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
UniProt ID: (Human) Q9NZQ7
Entrez Gene ID: (Human) 29126
adaptive immune response
immune system process
negative regulation of T cell mediated immune response to tumor cell
immune response
signal transduction
cell surface receptor signaling pathway
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
positive regulation of interleukin-10 production
response to cytokine
TRIF-dependent toll-like receptor signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of transcription, DNA-templated
regulation of activated T cell proliferation
negative regulation of activated T cell proliferation
negative regulation of T cell activation
regulation of T cell apoptotic process
cellular response to lipopolysaccharide
negative regulation of tumor necrosis factor superfamily cytokine production
regulation of activated CD4-positive, alpha-beta T cell apoptotic process
positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process
negative regulation of CD4-positive, alpha-beta T cell proliferation
negative regulation of CD8-positive, alpha-beta T cell activation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support