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Invitrogen
Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488
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FIGURE: 1 / 18
Occludin Antibody (331588) in ICC/IF
Knockout of Occludin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on Caco-2 wild type cells (panel a,d), Caco-2 Cas9 control cells (panel b,e) and Caco-2 Occludin KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488 (Product # 331588, 1:100). Nuclei (blue) were stained using ProLong™ Diamond Anti... View More
Product Details
331588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Bovine,
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
OC-3F10
Immunogen
GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 4.0mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tissues/lysates that were tested with this antibody include T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse), and rat liver.
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein; tight junction structural protein; unnamed protein product
Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115; PTORCH1
UniProt ID: (Human) Q16625, (Dog) Q28269, (Mouse) Q61146, (Rat) Q6P6T5
Entrez Gene ID: (Human) 100506658, (Dog) 403844, (Mouse) 18260, (Rat) 83497
positive regulation of lamellipodium assembly
positive regulation of gene expression
negative regulation of gene expression
epithelial cell migration
regulation of glucose transport
positive regulation of microtubule polymerization
maintenance of blood-brain barrier
cell-cell junction organization
positive regulation of glucose import
protein-containing complex assembly
bicellular tight junction assembly
positive regulation of wound healing
tight junction organization
protein localization to cell leading edge
positive regulation of maintenance of permeability of blood-brain barrier
methylation
S-adenosylhomocysteine metabolic process
S-adenosylmethionine metabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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