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Occludin Antibody (33-1500) in ICC/IF
Immunofluorescent analysis of Occludin was done on 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Occludin Mouse Monoclonal Antibody (Product # 33-1500) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 fo... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "Occludin Antibody (33-1500) in ICC/IF")
Product Details
33-1500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Bacteria,
Bovine,
C. elegans,
Chicken,
Cynomolgus monkey,
Dog,
Guinea pig,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Sheep,
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
OC-3F10
Immunogen
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: phosphatase 1, regulatory subunit 115; tight junction protein; tight junction structural protein; unnamed protein product
Gene Aliases: BLCPMG; Ocl; PPP1R115; PTORCH1
UniProt ID: (Human) Q16625, (Dog) Q28269, (Mouse) Q61146, (Rat) Q6P6T5
Entrez Gene ID: (Human) 100506658, (Dog) 403844, (Mouse) 18260, (Rat) 83497
positive regulation of lamellipodium assembly
positive regulation of gene expression
negative regulation of gene expression
epithelial cell migration
regulation of glucose transport
positive regulation of microtubule polymerization
maintenance of blood-brain barrier
cell-cell junction organization
positive regulation of glucose import
protein-containing complex assembly
bicellular tight junction assembly
positive regulation of wound healing
tight junction organization
protein localization to cell leading edge
positive regulation of maintenance of permeability of blood-brain barrier
response to interleukin-18
sensory perception of sound
response to ethanol
establishment of endothelial barrier
cellular response to tumor necrosis factor
response to Gram-positive bacterium
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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