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Occludin Antibody (740006M) in ICC/IF
Immunofluorescent analysis of Occludin was performed using 70% confluent Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10) (Product # 740006M, 1:2,000) at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32723) at a dilution of 1:2,000 for 1 hour at room temp... View More
Product Details
740006M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
OC-3F10
Immunogen
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin
Gene Aliases: BLCPMG; OCLN; PPP1R115
UniProt ID: (Human) Q16625
Entrez Gene ID: (Human) 100506658
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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