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Invitrogen
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488
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FIGURE: 1 / 31
ZO-1 Antibody (339188) in ICC/IF
Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488 at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin ... View More
Product Details
339188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Published species
Bovine,
C. elegans,
Dog,
Fish,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ZO1-1A12
Immunogen
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed. Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed.
For Alexa Fluor 488 excitation 495 nm/emission 519 nm. Each lot of this antibody is tested for specificity by immunofluorescence. Expires one year from date of receipt when stored as instructed.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Dog) O97758, (Human) Q07157
Entrez Gene ID: (Dog) 403752, (Human) 7082
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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