Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488 at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin ... View More

ZO-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ZO-1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ZO-1 Antibody — Figure 7 Induction of EMT after CPT treatment of SKOV3 cells A. RT-qPCR expression analysis of the indicated EMT marker genes after the indicated times of CPT treatment (sample size: n >= 3). B. Flow cytometry analysis of intracellular SMA after 0, 2 or 21 weeks of CPT treatment (sample size: n = 3). Values represent the fold change (FC) in the number of positive cells relative to small cells on day 0 (normalized to 1). Cells gated for small cells (forward and sideward scatter as untreated SKOV3 cells) and larger cells (forward and sideward scatter larger than for SKOV3 cells). C. Flow cytometry analysis of IL-1beta expression in SMA-expressing SKOV3-R cells gated as in panel B. D. Labeling of actin filaments with California Red-conjugated phalloidin (red) after different periods of CPT exposure. E. Additional staining of the tight junction (zonula occludens) protein constituent ZO-1 by indirect immunofluorescence (green) in untreated SKOV3 and SKOV3-R cells. Nuclei were stained with DAPI in panels D and E. F. RT-qPCR analysis of VIM expression in SKOV3-R cells (21w) and the same cells grown in the absence of CPT for 7 days. G. Pro-inflammatory and EMT marker gene expression after cyclic CPT treatment (1 day CPT, 21 days recovery, 4 cycles) compared to untreated cells (sample size: n = 3). {TM}

Immunogen image

Immunogen Image ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488

Product Details

339188

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 28 publications 28 publications

ELISA (ELISA)

0.1-1.0 µg/mL (unconjugated)

Product Specifications

Species Reactivity

Dog, Human

Published species

Bovine, Dog, Fish, Human, Mouse, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634

View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532187

Product Specific Information

This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed. Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed.

For Alexa Fluor 488 excitation 495 nm/emission 519 nm. Each lot of this antibody is tested for specificity by immunofluorescence. Expires one year from date of receipt when stored as instructed.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; unnamed protein product; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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Gene Aliases: TJP1; ZO-1; ZO1

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UniProt ID: (Human) Q07157, (Dog) O97758

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Entrez Gene ID: (Human) 7082, (Dog) 403752

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Function(s)

protein binding calmodulin binding cadherin binding cell adhesion molecule binding tight junction

Process(es)

cell-cell junction assembly positive regulation of cell proliferation actin cytoskeleton organization positive regulation of cell migration actomyosin structure organization adherens junction maintenance maintenance of blood-brain barrier ameloblast differentiation negative regulation of apoptotic process cell-cell junction organization regulation of cytoskeleton organization negative regulation of stress fiber assembly protein localization to adherens junction establishment of endothelial intestinal barrier cell-cell adhesion protein localization to cell-cell junction regulation of cell junction assembly protein localization to bicellular tight junction positive regulation of sprouting angiogenesis positive regulation of maintenance of permeability of blood-brain barrier positive regulation of cell-cell adhesion mediated by cadherin regulation of bicellular tight junction assembly bicellular tight junction assembly cellular response to calcium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488

ZO-1 Antibody (339188) in ICC/IF

Product Details

339188

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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