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Invitrogen
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 594
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FIGURE: 1 / 15
ZO-1 Antibody (339194) in ICC/IF
Immunofluorescence analysis of ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) (Product # 339194) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFa... View More
Product Details
339194
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Published species
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ZO1-1A12
Immunogen
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Conjugate
Excitation/Emission Max
590/618 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 4.0mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; unnamed protein product; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Human) Q07157, (Dog) O97758
Entrez Gene ID: (Human) 7082, (Dog) 403752
cell-cell junction assembly
positive regulation of cell proliferation
actin cytoskeleton organization
positive regulation of cell migration
actomyosin structure organization
adherens junction maintenance
maintenance of blood-brain barrier
ameloblast differentiation
negative regulation of apoptotic process
cell-cell junction organization
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
protein localization to adherens junction
establishment of endothelial intestinal barrier
cell-cell adhesion
protein localization to cell-cell junction
regulation of cell junction assembly
protein localization to bicellular tight junction
positive regulation of sprouting angiogenesis
positive regulation of maintenance of permeability of blood-brain barrier
positive regulation of cell-cell adhesion mediated by cadherin
regulation of bicellular tight junction assembly
bicellular tight junction assembly
cellular response to calcium ion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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