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Invitrogen
ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 594
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FIGURE: 1 / 15
ZO-1 Antibody (339194) in ICC/IF
Immunofluorescence analysis of ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) (Product # 339194) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
339194
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Published species
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ZO1-1A12
Immunogen
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Conjugate
Alexa Fluor™ 594
Alexa Fluor™ 594
Alexa Fluor™ 594
Excitation/Emission Max
590/618 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 4.0mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; Zona occludens protein 1; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Dog) O97758
Entrez Gene ID: (Dog) 403752
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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