Mouse IFN gamma Recombinant Protein

Product Specific Information

Description: Mouse IFN gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: Recombinant mouse IFN gamma is biologically active and is for bioassay use only. For an ELISA standard, please refer to eBioscience Cat. No. 39-8311-65.

Applications Tested: The ED50 of this protein, as measured by EMC virus protection assay with L929 cells, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Source: E. coli expressed amino acids His 23-Cys 155 of mouse IFN gamma accession # NM_008337.

Bioactivity: The ED50 of this protein, as measured by EMC virus protection assay with L929 cells, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Molecular Weight: The protein is methionylated at the N-terminal. The DTT reduced and protein migrates as a 14.5 kDa polypeptides on SDS-PAGE.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.