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TCR V delta 1 Monoclonal Antibody (TS8.2), Brilliant Violet™ 605, eBioscience™
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FIGURE: 1 / 1
TCR V delta 1 Antibody (406-5679-42) in Flow
Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0038-42), TCR gamma/delta Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9959-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 605 (Product # 406-4714-81) (left) or TCR V delta 1 Monoclonal Antibody, Brilliant Violet 605 (right). Viable CD3 positive cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
406-5679-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TS8.2
Immunogen
Full length protein of Human TCR delta v1
Conjugate
Excitation/Emission Max
406/603 nm
View spectra
Form
liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Description
This TS8.2 monoclonal antibody recognizes human TCR V delta 1, a common framework determinant on the delta-subunit of the T cell receptor. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity. TCR V delta 1 Monoclonal Antibody (TS8.2) has been validated for the following applications Flow Cytometry
Applications Tested
This TS8.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
The T Cell Receptor gamma-delta (TCR gamma delta) is a heterodimeric membrane receptor that consists of the highly variable gamma and delta chains expressed as part of a complex with the invariant CD3 proteins. Gamma-delta T cells are less frequent than alpha-beta T cells but compose a substantial part of T cells found at epithelial borders including the gut mucosa resident intraepithelial lymphocytes (IEL). The antigenic stimuli required for gamma-delta T cell receptor activation are not well understood and are thought to be mainly MHC class-independent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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