Invitrogen

IL-4 Monoclonal Antibody (11B11), Brilliant Violet™ 480, eBioscience™

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Product Details

414-7041-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 480, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11B11

Conjugate

Brilliant Violet™ 480 Brilliant Violet™ 480 Brilliant Violet™ 480

Excitation/Emission Max

437/478 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074107

Product Specific Information

Description
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Tested
This 11B11 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse Th2-polarized splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.

Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)
cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily
Process(es)
microglial cell activation positive regulation of protein phosphorylation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment positive regulation of immunoglobulin production negative regulation of acute inflammatory response negative regulation of chronic inflammatory response immune response cholesterol metabolic process positive regulation of cell proliferation regulation of proton transport negative regulation of epithelial cell migration positive regulation of B cell proliferation B cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-13 production T-helper 2 cell cytokine production positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan negative regulation of macrophage activation positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation positive regulation of chemokine biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response negative regulation of T cell activation positive regulation of B cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast fusion negative regulation of complement-dependent cytotoxicity negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis negative regulation of extrinsic apoptotic signaling pathway
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