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          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Monoclonal Antibody (MAb11), Brilliant Violet™ 650, eBioscience™

          View all (221) TNF alpha antibodies

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          Cite TNF alpha Monoclonal Antibody (MAb11), Brilliant Violet™ 650, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          TNF alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          TNF alpha Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          TNF alpha Antibody (416-7349-42) in Flow

          Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG1 kappa Isotype Control, Brilliant Violet 650 (Product # 416-4714-81) (blue histogram) or TNF alpha Monoclonal Antibody, Brilliant Violet 650 (purple histogram). Viable cells in the lymphocyte gate we... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          TNF alpha Antibody in Flow Cytometry (Flow)

          Product Details

          416-7349-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (1.0 µg)/test
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 650, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          MAb11

          Conjugate

          Brilliant Violet™ 650 Brilliant Violet™ 650 Brilliant Violet™ 650
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • eFluor 450
          • FITC
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Request custom conjugation

          Excitation/Emission Max

          407/646 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2925712

          Product Specific Information

          Description
          The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

          Applications Tested
          This MAb11 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Blocking Buffers
          When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

          Light sensitivity
          This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation
          • Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
          • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
          • Our internal testing suggests that Brilliant Violet™ 650 (BV650) is not compatible with methanol-based fixation.

          Excitation: 407 nm; Emission: 649 nm; Laser: Violet Laser.

          BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

          Target Information

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: APC1 protein; Cachectin; cell apoptosis-related protein; DADB-70P7.1; TNF alpha; TNF superfamily; TNF superfamily member 2; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrotic factor alpha; unnamed protein product

          View more View less

          Gene Aliases: DIF; IMD127; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

          View more View less

          UniProt ID: (Human) P01375

          View more View less

          Entrez Gene ID: (Human) 7124

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding protease binding cytokine activity tumor necrosis factor receptor binding protein binding death receptor agonist activity identical protein binding receptor agonist activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter response to hypoxia microglial cell activation positive regulation of cytokine production positive regulation of protein phosphorylation negative regulation of endothelial cell proliferation negative regulation of L-glutamate transport macrophage activation involved in immune response chronic inflammatory response to antigenic stimulus regulation of immunoglobulin production positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin skeletal muscle contraction negative regulation of systemic arterial blood pressure glucose metabolic process defense response inflammatory response immune response humoral immune response JNK cascade JAK-STAT cascade circadian rhythm negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus response to mechanical stimulus response to virus response to bacterium response to salt stress response to fructose organ morphogenesis negative regulation of heart rate vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation negative regulation of lipid storage response to activity regulation of metabolic process calcium-mediated signaling extracellular matrix organization osteoclast differentiation positive regulation of protein complex assembly positive regulation of defense response positive regulation of fever generation negative regulation of myelination response to nutrient levels response to lipopolysaccharide negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of fractalkine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion toll-like receptor 3 signaling pathway positive regulation of cellular amide metabolic process response to isolation stress response to macrophage colony-stimulating factor NIK/NF-kappaB signaling regulation of cell proliferation vasodilation defense response to bacterium positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of MAP kinase activity positive regulation of MAPK cascade protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration negative regulation of viral genome replication innate immune response cellular extravasation positive regulation of nitric oxide biosynthetic process response to ethanol regulation of fat cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of protein catabolic process positive regulation of action potential positive regulation of cell adhesion positive regulation of lipid metabolic process positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade astrocyte activation embryonic digestive tract development positive regulation of smooth muscle cell proliferation regulation of protein secretion regulation of inflammatory response positive regulation of inflammatory response negative regulation of neurogenesis regulation of developmental process regulation of insulin secretion modulation of synaptic transmission positive regulation of synaptic transmission regulation of synapse organization defense response to Gram-positive bacterium cognition leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis regulation of secretion positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport regulation of multicellular organismal process response to glucocorticoid positive regulation of hair follicle development positive regulation of protein kinase B signaling regulation of synaptic transmission, glutamatergic positive regulation of glial cell proliferation positive regulation of vitamin D biosynthetic process epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of vascular wound healing negative regulation of branching involved in lung morphogenesis regulation of biological quality positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to retinoic acid cellular response to nicotine cellular response to interferon-gamma cellular response to ionizing radiation positive regulation of mononuclear cell migration positive regulation of podosome assembly endothelial cell apoptotic process protein localization to plasma membrane negative regulation of oxidative phosphorylation inflammatory response to wounding apoptotic signaling pathway extrinsic apoptotic signaling pathway cellular response to toxic substance liver regeneration necroptotic signaling pathway negative regulation of bile acid secretion antiviral innate immune response response to Gram-negative bacterium positive regulation of neuroinflammatory response positive regulation of interleukin-33 production positive regulation of cytokine production involved in inflammatory response negative regulation of beta-amyloid clearance positive regulation of NIK/NF-kappaB signaling positive regulation of synoviocyte proliferation positive regulation of beta-amyloid formation response to L-glutamate positive regulation of neutrophil activation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of cellular protein localization cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell regulation of membrane lipid metabolic process response to 3,3',5-triiodo-L-thyronine response to gold nanoparticle positive regulation of protein localization to cell surface positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of hepatocyte proliferation regulation of endothelial cell apoptotic process regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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