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Invitrogen

CD36 Monoclonal Antibody (eBioNL07 (NL07)), PerCP-eFluor™ 710, eBioscience™

4 Published Figures
6 References
View all (88) CD36 antibodies

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Product Details

46-0369-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 6 publications 6 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioNL07 (NL07)

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220281

Product Specific Information

Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.

Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral bloocd cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: 85 kda protein; 88 kda cell surface glycoprotein; CD36; CD36 antigen; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (CD36 blood group) transcript; CD36 molecule (thrombospondin receptor); CD36 molecule (thrombospondin receptor) isoform CD36_1; CD36 molecule (thrombospondin receptor) isoform CD36_2; CD36 molecule (thrombospondin receptor) isoform CD36_3; cluster determinant 36; FAT; Fatty acid translocase; glycoprotein GPIIIb/GPIV; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor; unnamed protein product

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Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

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UniProt ID: (Human) P16671

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Entrez Gene ID: (Human) 948

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Function(s)
beta-amyloid binding low-density lipoprotein receptor activity scavenger receptor activity long-chain fatty acid transporter activity protein binding high-density lipoprotein particle binding lipid binding short-chain fatty acid uptake transporter activity low-density lipoprotein particle binding Toll-like receptor binding cargo receptor activity macromolecular complex binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding oxidised low-density lipoprotein particle receptor activity oleate transmembrane transporter activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade positive regulation of cell-matrix adhesion production of molecular mediator involved in inflammatory response lipid metabolic process fatty acid metabolic process lipid transport receptor-mediated endocytosis phagocytosis, recognition phagocytosis, engulfment cell adhesion cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration blood coagulation response to bacterium positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol storage fatty acid transport long-chain fatty acid transport plasma membrane long-chain fatty acid transport short-chain fatty acid transport negative regulation of angiogenesis lipid storage cGMP-mediated signaling positive regulation of blood coagulation intestinal cholesterol absorption cholesterol transport receptor internalization regulation of lipopolysaccharide-mediated signaling pathway positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production response to lipid regulation of toll-like receptor signaling pathway triglyceride transport plasma lipoprotein particle clearance low-density lipoprotein particle clearance cellular response to oxidative stress response to stilbenoid nitric oxide-cGMP-mediated signaling pathway negative regulation of protein import into nucleus lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly apoptotic cell clearance positive regulation of MAPK cascade long-chain fatty acid import positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis defense response to Gram-positive bacterium intestinal absorption sensory perception of taste low-density lipoprotein particle mediated signaling positive regulation of phagocytosis, engulfment positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade cholesterol import response to fatty acid response to linoleic acid cellular response to bacterial lipopeptide cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to low-density lipoprotein particle stimulus cellular response to hydroperoxide cellular response to diacyl bacterial lipopeptide energy homeostasis regulation of action potential positive regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus oxidised low-density lipoprotein particle clearance amyloid-beta clearance by cellular catabolic process positive regulation of NLRP3 inflammasome complex assembly positive regulation of protein localization to plasma membrane positive regulation of reactive oxygen species biosynthetic process cellular response to beta-amyloid amyloid fibril formation lipid transport across blood brain barrier regulation of removal of superoxide radicals positive regulation of blood microparticle formation positive regulation of reactive oxygen species metabolic process
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