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Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), eFluor™ 450, eBioscience™

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12 Published Figures
11 References
View all (140) IL-2 antibodies

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Product Details

48-7029-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test
View 11 publications 11 publications
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574101

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: This MQ1-17H12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ1-17H12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)
cytokine activity interleukin-2 receptor binding protein binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding
Process(es)
adaptive immune response leukocyte activation involved in immune response immune system process positive regulation of immunoglobulin production negative regulation of B cell apoptotic process transcription from RNA polymerase II promoter immune response cell adhesion G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling positive regulation of cell proliferation gene expression natural killer cell activation T cell differentiation positive regulation of cell growth positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-17 production positive regulation of tissue remodeling interleukin-2-mediated signaling pathway T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of apoptotic process response to ethanol positive regulation of T cell differentiation positive regulation of regulatory T cell differentiation positive regulation of lymphocyte differentiation positive regulation of transcription from RNA polymerase II promoter regulation of T cell homeostatic proliferation lymphocyte proliferation positive regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of inflammatory response positive regulation of inflammatory response activated T cell proliferation positive regulation of dendritic spine development extrinsic apoptotic signaling pathway in absence of ligand receptor signaling pathway via STAT positive regulation of plasma cell differentiation response to tacrolimus negative regulation of T-helper 17 cell differentiation regulation of CD4-positive, alpha-beta T cell proliferation
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