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Invitrogen
IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™
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FIGURE: 1 / 14
IL-2 Antibody (14-7029-81) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and 0.125 µg IL-2 Monoclonal Antibody followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Cells in the lymphocyte gate were used for analysis.
Product Details
14-7029-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cynomolgus monkey,
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
MQ1-17H12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.
Applications Reported: The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis. Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry.
Applications Tested: The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (13-7028) antibody for detection and recombinant human IL-2 (14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
adaptive immune response
leukocyte activation involved in immune response
immune system process
positive regulation of immunoglobulin production
negative regulation of B cell apoptotic process
transcription from RNA polymerase II promoter
immune response
cell adhesion
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
positive regulation of cell proliferation
gene expression
natural killer cell activation
T cell differentiation
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of tissue remodeling
interleukin-2-mediated signaling pathway
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
negative regulation of apoptotic process
response to ethanol
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
positive regulation of lymphocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
lymphocyte proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of inflammatory response
activated T cell proliferation
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
receptor signaling pathway via STAT
positive regulation of plasma cell differentiation
response to tacrolimus
negative regulation of T-helper 17 cell differentiation
regulation of CD4-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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