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Cytokeratin 19 Antibody Cocktail
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FIGURE: 1 / 5
Cytokeratin 19 Antibody (61010PROGEN) in IHC (P)
Immunohistochemistry analysis of human head and neck squamous cell carcinoma using anti-Keratin K19 antibody (Cat. No. 690010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary anti... View More
Product Details
61010PROGEN
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Cocktail
Type
Antibody
Clone
Ks19.1 (A53-B/A2)
Immunogen
Keratin K19 Of Mr 40 000; From Cultured Human Mcf-7 Cells
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute lyophilized product with 1 mL distilled water.
Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Polypeptide reacting: MW 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the alpha -helical fragment.Tumors specifically detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma. Reactivity on cultured cell lines: MCF-7. This antibody was used in a Sandwich ELISA, and pairs with products 61029, 690029, and 690029S.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366; unnamed protein product
Gene Aliases: CK19; K19; K1CS; KRT19
UniProt ID: (Human) P08727
Entrez Gene ID: (Human) 3880
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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