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PKM2 Antibody (703382) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of PANC-1 (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4) and SK-BR-3 (Lane 5). The blot was probed with Anti-PKM2 Recombinant Rabbit Monoclonal Antibody (Product # 703382, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~58 kDa band corresponding to PKM2 was observed across the cell lines tested.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
703382
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3H4L19
Immunogen
Peptides corresponding to human PKM2 [(aa476-aa493) (aa120-aa136)]
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Pig, Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic thyroid hormone-binding protein; cytosolic thyroid hormone-binding protein (EC 2.7.1.40); epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OPA-interacting protein 3; PK, muscle type; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase, muscle; similar to human pyruvate kinase M2; threonine-protein kinase PKM2; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; tumor M2-PK; tyrosine-protein kinase PKM2; unnamed protein product
Gene Aliases: CTHBP; HEL-S-30; OIP3; p58; PK3; PKM2; TCB; THBP1
UniProt ID: (Human) P14618
Entrez Gene ID: (Human) 5315
magnesium ion binding
transcription coactivator activity
RNA binding
mRNA binding
protein serine/threonine kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
pyruvate kinase activity
protein binding
MHC class II protein complex binding
potassium ion binding
histone kinase activity (H3-T11 specific)
protein homodimerization activity
cadherin binding
glycolytic process
chromatin remodeling
programmed cell death
cellular response to insulin stimulus
pyruvate biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
canonical glycolysis
positive regulation of sprouting angiogenesis
positive regulation of cytoplasmic translation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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